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Merck
CN

P1598

Sigma-Aldrich

PCR 20 bp Low Ladder

for electrophoresis of PCR fragments

Synonym(s):

20 bp Low Ladder, Marker for DNA gel electrophoresis

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About This Item

UNSPSC Code:
41141827
NACRES:
NA.25

Key Documents

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biological source

fermentation/recombinant (E. coli strain DH5A)

Quality Level

100

grade

Molecular Biology

form

liquid

usage

80 uses

solubility

water: soluble at 20 °C

suitability

suitable for electrophoresis (DNA)

storage temp.

2-8°C

Related Categories

General description

Sigma′s PCR 20 bp Low Ladder contains 50 dsDNA fragments from 50 – 1,000 bases, evenly spaced in 20 bp repeats. Five μl of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose gel.

Application

PCR 20 bp Low Ladder has been used as a marker for DNA gel electrophoresis.

Other Notes

Contains 50 bands, 20–1,000 bp in exact 20 bp repeats.
For optimal resolution, the recommended agarose gel concentration is 2.5%.
Sigma′s PCR 20 bp Low Marker is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

常规特殊物品
This item has

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000500137
0000500137
0000284868
0000265950
SLCR4827

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Primary mediastinal B-cell lymphoma: detection of BCL2 gene rearrangements by PCR analysis and FISH.
Dunphy CH, et al.
Journal of Hematopathology, 1, 77-84 (2008)

Articles

Selecting DNA, RNA, and PCR Fragment Markers and Ladders for Gel Electrophoresis

Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.

Gel Electrophoresis Markers

Markers for gel electrophoresis aid size determination of DNA, PCR fragments, and RNA, staining well with common nucleic acid stains.

选择用于凝胶电泳的DNA、RNA和PCR片段的标记物和分子量标准

选择适合的标记物和分子量标准，用于测定电泳分离样品的核酸大小。通过琼脂糖或聚丙烯酰胺凝胶检测DNA、RNA和PCR生成片段的大小。

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