P2297
Picrylsulfonic acid solution
5 % (w/v) in H2O, BioReagent, suitable for determination of primary amines
product line
BioReagent
Quality Level
concentration
5 % (w/v) in H2O
suitability
suitable for determination of primary amines
storage temp.
2-8°C
SMILES string
OS(=O)(=O)c1c(cc(cc1[N+]([O-])=O)[N+]([O-])=O)[N+]([O-])=O
InChI
1S/C6H3N3O9S/c10-7(11)3-1-4(8(12)13)6(19(16,17)18)5(2-3)9(14)15/h1-2H,(H,16,17,18)
InChI key
NHJVRSWLHSJWIN-UHFFFAOYSA-N
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Related Categories
General description
Application
- to assess the efficiency of carboxymethyl lysine (CML) modifications of exposed primary amines
- to incubate zona intact bovine embryos for the differential staining of blastocysts
- to induce irritable bowel syndrome (IBS) in mice and to study its effects on Clostridium butyricum
related product
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Dam. 1 - Skin Corr. 1B - Skin Sens. 1
Storage Class Code
8B - Non-combustible, corrosive hazardous materials
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Is Product P2297, Picrylsulfonic acid solution, supposed to be yellow?
Yes. This product is a clear to slightly hazy yellow solution at room temperature. The solution has a concentration of 5 w/v % in H2O (5g per 100 mL).
In general, what is Product P2297, Picrylsulfonic acid solution, used for?
Picrylsulfonic acid has been used 1) As a hydrophilic modifying reagent[1]; 2) To make derivatives with amino compounds, which can be regenerated with hydrazine [2].
How is Product P2297, Picrylsulfonic acid solution, tested? Do you have any references for its use besides Sigma's method?
Sigma's suitability assay is based on a published method.1 We use a 500-fold dilution as a working solution, with glycine as a standard. Hydrochloric acid is used to stop the reaction, and to reduce yellow background due to picric acid. Sodium phosphate is used as a buffer in place of sodium bicarbonate to avoid formation of CO2 when HCl is added. Absorbance is read at 420 nm.Other procedures have been published. For example:---Twenty-five mL of 0.03 M TNBS is added to 1 mL of sample (in borate buffer), agitated to ensure complete mixing, and allowed to stand for 30 minutes at room temperature (25±1°C). No quenching step is used. In the blank, buffer replaces sample. Absorbance is read at 420 nm.2---Protocols using a more concentrated solution of picric acid (1.1 M) have been published.3Note: TNBS forms an orange complex with amines, monitored at 420 nm, but the EmM of the complexes will vary with the reacting group: for alpha-amino groups, 22.0; for epsilon-amino groups, somewhat lower; and for thiol groups, 2.25.3References:1. Satake, K. et al., J. Biochem., 47, 654 (1960).2. Snyder, S.L. and Sobocinski P.Z., Anal. Biochem., 64, 284-288 (1975).3. Fields, R., Methods in Enzymology, 25, 464-468 (1972).
What are the suggested storage conditions for Product P2297, Picrylsulfonic acid solution?
Store Product No. P2297 at 2-8°C in the dark.
Is Product P2297, Picrylsulfonic acid solution, considered to be stable?
It is explosive when it is dry. The solution should be stored at 2-8°C in the dark. Under these conditions less than 5% decomposition will occur over five months. At 37°C, 50% decomposition occurred in 60 days. Picrylsulfonic acid decomposes to picric acid, crystals of which may form in the solution, but this impurity causes no interference in usage. The solution may also contain a trace amount of 2,4-dinitrophenol (typically <0.5%). Storage at -20°C may enhance shelf-life of stock solutions.
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