P2545
Protein A–Agarose
saline suspension
Synonym(s):
Protein A resin
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About This Item
Form:
saline suspension
Biological source:
Staphylococcus aureus
biological source
Staphylococcus aureus
Quality Segment
form
saline suspension
extent of labeling
~2 mg per mL
matrix activation
cyanogen bromide
matrix attachment
amino
matrix spacer
1 atom
capacity
≥15 mg/mL binding capacity (human IgG)
storage temp.
2-8°C
General description
Protein A is a 42-kDa protein from Staphylococcus aureus and has five immunoglobulin binding domains.
Application
Protein A−Agarose has been used in the immunoprecipitation
- of protein phosphatase and tensin homolog (PTEN) protein from S63 and NIH3T3 fibroblast cells
- of prolyl hydroxylase domain 2 N27 cells
- of Wnt3a and sonic hedgehog (Shh) in mouse fibroblast L-cells and human embryonic kidney (HEK) 293 cells
Protein A-agarose is used for affinity chromatography, antibody purification and characterization, and protein A, G and L resins. Protein A-agarose has been used to study the effects of protein A immunoadsorption in patients with chronic dilated cardiomyopathy as well as to study multiple sclerosis and gastric cancer.
Biochem/physiol Actions
Protein A binds to Fcγ (the constant region) and Fab (antigen binding fragment) of IgG. Protein A agarose resin is commercially used for affinity based purifications.
Physical form
Suspension in 0.5 M NaCl containing preservative
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Storage Class
10 - Combustible liquids
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
signalword
Warning
hcodes
Hazard Classifications
Skin Sens. 1
Regulatory Information
常规特殊物品
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Related Content
EZviewTM Red Protein A and ANTI-FLAG® M2 Affinity Gels: Immunoprecipitation with Enhanced Visibility Affinity Beads - Technical Article - July 2001
Global Trade Item Number
| SKU | GTIN |
|---|---|
| P2545-5ML | 04061834364009 |
| P2545-1ML | 04061834363996 |