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P2636

Sigma-Aldrich

Poly-L-lysine hydrobromide

suitable for cell culture and bioconjugation, Mol wt 30,000-70,000

Synonym(s):

L-Lysine homopolymer hydrobromide

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Estimated to ship onOctober 09, 2025Details


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1 EA
CN¥6,384.05

About This Item

CAS Number:
MDL number:
UNSPSC Code:
12352209
eCl@ss:
32160406
PubChem Substance ID:
NACRES:
NA.26

CN¥6,384.05


Estimated to ship onOctober 09, 2025Details


Request a Bulk Order

Product Name

Poly-L-lysine hydrobromide, mol wt 30,000-70,000

form

powder

Quality Level

mol wt

30,000-70,000

technique(s)

bioconjugation: suitable
cell culture | mammalian: suitable

impurities

<10% water (Karl Fischer)

color

white to faint yellow

solubility

water: 50 mg/mL, clear, colorless to light yellow (50 mg/mL, water, Clear, Colorless to light yellow
)

functional group

amine
carboxylic acid

storage temp.

−20°C

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This Item
GF27798485GF34555215GF05036509
镍 wire reel, 5m, diameter 0.0125mm, Stress relieved, 99.98%

GF33022381

镍 wire reel, 10m, diameter 0.0125mm, Stress relieved, 99.98%

GF27798485

镍 wire reel, 5m, diameter 0.25mm, annealed, 99%

GF34555215

镍 wire reel, 5m, diameter 0.5mm, annealed, 99%

GF05036509

manufacturer/tradename

Goodfellow 330-223-81

manufacturer/tradename

Goodfellow 277-984-85

manufacturer/tradename

Goodfellow 345-552-15

manufacturer/tradename

Goodfellow 050-365-09

form

wire

form

wire

form

wire

form

wire

assay

99.98%

assay

99.98%

assay

99%

assay

99%

resistivity

6.97 μΩ-cm, 20°C

resistivity

6.97 μΩ-cm, 20°C

resistivity

6.97 μΩ-cm, 20°C

resistivity

6.97 μΩ-cm, 20°C

density

8.9 g/mL at 25 °C (lit.)

density

8.9 g/mL at 25 °C (lit.)

density

8.9 g/mL at 25 °C (lit.)

density

8.9 g/mL at 25 °C (lit.)

bp

2732 °C (lit.)

bp

2732 °C (lit.)

bp

2732 °C (lit.)

bp

2732 °C (lit.)

General description

Poly-L-lysine serves as a versatile and indispensable component in cell culture and biochemical research. It plays a pivotal role in promoting cell adhesion to solid substrates by enhancing the electrostatic interactions between the negatively charged ions of cell membranes and the positively charged ions of attachment factors on culture surfaces. This facilitates a secure binding of cells to the substratum, making Poly-L-lysine highly recommended for cell culture applications.

Derived from the naturally occurring amino acid l-lysine, Poly-L-lysine is available in various forms, allowing for customization in terms of molecular weight and shape. As a synthetic positively charged polymer, it exists in two enantiomers, poly-D-lysine and poly-L-lysine, both of which have their unique applications.

In formulation research, Poly-L-lysine proves to be valuable in nucleic acid delivery. Its positive charge, attributed to the ε-amine on its side chain at physiological pH, enables it to condense plasmid DNA effectively based on salt concentration. While Poly-L-lysine appears suitable for gene delivery, unmodified versions have been associated with low transfection efficiency and cytotoxicity.

In conclusion, Poly-L-lysine offers a multifaceted solution in cell culture and formulation research, enhancing cell adhesion, enabling gene delivery, and contributing significantly to advancements in biochemical and cell biology research.

Application

Poly-L-lysine polymers can be used in promoting cell adhesion to solid substrates, conjugation to methotrexate for increased drug transport, microencapsulation of islets, cell microencapsulation technology, microarray glass slide coating, and chromosomal preparations. Lower molecular weight poly-L-lysine (30,000-70,000) is less viscuous in solution, but higher molecular weight versions provide more attachment sites per molecule.

Biochem/physiol Actions

Poly-L-lysine is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. When it is absorbed to the cell culture surface, poly-L-lysine functions to increase the number of positively charged sites available for cell binding. With cells that can digest poly-L-lysine, poly-D-lysine should be used as the attachment factor.

Features and Benefits

  • Can be used in Metabolomics and Biochemical research
  • High-quality compound suitable for multiple research applications

Components

Poly-L-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-L-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the beta structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties.

Caution

Sterile solutions are stable for up to 2 years when stored at 2-8°C. It should be stored desiccated at -20°C.

Preparation Note

This product has a molecular weight of 30,000-70,000. To remove the HBr, dissolve this product in a neutral buffer and dialyze to remove the salts. None of the poly-L-lysine products have been exposed to trifluoroacetic acid and are dialyzed to remove any monomers, dimmers, or trimers, confirmed by thin layer chromatography. In general, to use this product as an attachment factor, add 50 mL of sterile tissue culture grade water to 5 mg of poly-lysine, and aseptically coat the surface with 1 mL per 25 cm2 of solution. After 5 minutes, remove the solution through aspiration and thoroughly rinse the surface. Let dry for two hours before introducing cells and medium.

Analysis Note

Molecular weight based on viscosity and was also assayed by MALLS.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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    1. How does the storage temperature relate to shipping conditions?

      The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment. See Shipping and Storage for more information.

    2. Which document(s) contains shelf-life or expiration date information for a given product?

      If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

    3. How do I get lot-specific information or a Certificate of Analysis?

      The lot specific COA document can be found by entering the lot number above under the "Documents" section.

    4. How do I find price and availability?

      There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

    5. What is the Department of Transportation shipping information for this product?

      Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

    6. What is the average particle size of Product 683043, Magnesium hydride?

      According to our supplier, the average particle size is approximately 50 microns.

    7. What is the bulk density for Product 683043, Magnesium hydride?

      According to our supplier, the bulk density is approximately 0.5 g/cm³.

    8. At what temperature will magnesium hydride start to decompose?

      Magnesium hydride has a decomposition temperature of 284°C.

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