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About This Item
NACRES:
NA.46
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
SPA-27, monoclonal
Application:
ELISA (i), ICC, WB
Citations:
19
biological source
mouse
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
SPA-27, monoclonal
contains
15 mM sodium azide
technique(s)
immunocytochemistry: suitable, indirect ELISA: 1:20,000, western blot: suitable
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
General description
Protein A is a 42 kDa single chain polypeptide isolated from the cell wall of Staphylococcus aureus Cowan I strain.
Immunogen
protein A from Cowan I strain of Staphylococcus aureus.
Application
Monoclonal Anti-Protein A antibody produced in mouse has been used in enzyme-linked immunosorbent assay (ELISA). It has also been used in western blot and dot blot analyses.
Biochem/physiol Actions
Protein A is considered as a universal reagent in biochemistry and immunology, due to its affinity for the Fc region of many mammalian immunoglobulins. It is used for different applications such as purification of immunoglobulins by affinity chromatography, cell surface studies, radioimmunoassay (RIA), enzyme immunoassay (EIA), immunoprecipitations and many other procedures. It can be used in these methods either in its native form or conjugated to various markers.
Specific for Protein A and shows no cross-reactivity with Protein G. Reacts with free Protein A or IgG bound Protein A and does not interfere with the Fc binding activity of Protein A.
Physical form
Monoclonal Anti-Protein A antibody produced in mouse is provided as ascites fluid with 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
常规特殊物品
动植物来源生物产品
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Fabien Bonneau et al.
Genes & development, 37(11-12), 505-517 (2023-07-03)
Messenger RNAs (mRNAs) are at the center of the central dogma of molecular biology. In eukaryotic cells, these long ribonucleic acid polymers do not exist as naked transcripts; rather, they associate with mRNA-binding proteins to form messenger ribonucleoprotein (mRNP) complexes.
Sandra K Schuller et al.
eLife, 9 (2020-11-17)
The yeast THO complex is recruited to active genes and interacts with the RNA-dependent ATPase Sub2 to facilitate the formation of mature export-competent messenger ribonucleoprotein particles and to prevent the co-transcriptional formation of RNA:DNA-hybrid-containing structures. How THO-containing complexes function at
Shan Huang et al.
Frontiers in molecular biosciences, 7, 581095-581095 (2021-01-12)
Hog1 is a mitogen-activated protein kinase in yeast that primarily regulates cellular responses to hyperosmolarity stress. In this study, we have examined the potential involvement of Hog1 in mediating cellular responses to DNA damaging agents. We find that treatment of
A simple and robust method for the complete dissociation of HIV-1 p24 and other antigens from immune complexes in serum and plasma samples
Steindl F, et al.
Journal of Immunological Methods, 217(1-2), 143-151 (1998)
Francisco J Navarro et al.
Journal of cell science, 130(24), 4144-4154 (2017-11-01)
Sexual differentiation in the fission yeast
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