P302-05
Porcine Pulmonary Artery Endothelial Cells: PPAEC (Cryovial)
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About This Item
NACRES:
NA.81
UNSPSC Code:
41106514
Biological source:
Porcine pulmonary artery (Normal healthy)
Growth mode:
Adherent
Morphology:
Endothelial
Relevant disease(s):
diabetes; cardiovascular diseases
biological source
Porcine pulmonary artery (Normal healthy)
form
solid
packaging
pkg of 500,000 cells
manufacturer/tradename
Cell Applications, Inc
growth mode
Adherent
karyotype
2n = 38
morphology
Endothelial
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
diabetes; cardiovascular diseases
shipped in
dry ice
storage temp.
−196°C
Quality Level
General description
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PPAEC have been used in a pioneer study of endothelial cell motility, where it was determined that asymmetric dimethylarginine (ADMA), an inhibitor of nitric oxide production and a cardiovascular risk factor, decreased PKG-mediated phosphorylation of VASP, leading to downregulation of Rac1 and induction of stress fibers and focal adhesions via RhoA/ROCK dependent mechanism, leading to decreased cell motility, and increase in endothelial permeability, thus linking vascular repair and function with NO production (Wojciak-Stothard, 2007, 2009). PPAEC were also used to assess endothelial adhesion to decellularized porcine arteries in order to develop grafts for human vascular repair (Xiong, 2013).
PPAEC have been used in a pioneer study of endothelial cell motility, where it was determined that asymmetric dimethylarginine (ADMA), an inhibitor of nitric oxide production and a cardiovascular risk factor, decreased PKG-mediated phosphorylation of VASP, leading to downregulation of Rac1 and induction of stress fibers and focal adhesions via RhoA/ROCK dependent mechanism, leading to decreased cell motility, and increase in endothelial permeability, thus linking vascular repair and function with NO production (Wojciak-Stothard, 2007, 2009). PPAEC were also used to assess endothelial adhesion to decellularized porcine arteries in order to develop grafts for human vascular repair (Xiong, 2013).
Application
cardiovascular function, screening of drug candidates, angiogenesis, vasodilator function, transport of deoxygenated bloood from heart to lungs
Biochem/physiol Actions
Artery
Preparation Note
- 1st passage, >500,000 cells in Porcine Endothelial Cell Growth Medium that contains 10% FBS and 10% DMSO
- Can be cultured at least 16 doublings
Please refer to the PPAEC Culture Protocol.
Other Notes
Porcine Endothelial Cell Growth Medium that contains 10% FBS and 10% DMSO
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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