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Merck
CN

P3288

Phosphate buffer pH 7.2 at 25 °C

for hematology and histology staining techniques

Synonym(s):

phosphate buffer

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About This Item

NACRES:
NA.47
UNSPSC Code:
12352107
Technical Service
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Quality Level

form

powder

color

white

pH

7.10-7.30

application(s)

hematology
histology

storage temp.

room temp

General description

Phosphate buffer is the most commonly used component of irrigation solution during surgeries, to preserve the living tissues. It is also used in immunochemical staining procedures. Phosphate buffer serves as an important fixative for light and electron microscopy. Its buffering capacity is pH sensitive and is preferred to be used at physiological pH (7.2-7.4).

Application

Intended for use as a buffer in various Romanowsky type staining procedures, including Wright Stain, Wright Giemsa, Giemsa, May Grunwald, Jenner and Leishman. Phosphate buffer pH 7.2 at 25 °C has been used in oocyte collection from porcine ovaries.

Preparation Note

Each vial will prepare 3.8 L of buffer, 8.3 mM.


Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable



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Learn about the clinical study of blood, blood-forming organs, and blood diseases including the treatment, prevention, and stains and dyes used in hematology testing.


Biological Electron Microscopy: Theory, Techniques, and Troubleshooting (2011)
Low concentrations of MEM vitamins during in vitro maturation of porcine oocytes improves subsequent parthenogenetic development
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Global Trade Item Number

SKUGTIN
P3288-1VL04061834367604
P3288-12VL04061834367598