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P3369

Sigma-Aldrich

Protamine sulfate

meets USP testing specifications

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Synonym(s):
Salmine sulfate from salmon sperm, Protamine sulfate
MDL number:
MFCD00132091
NACRES:
NA.77

Agency

meets USP testing specifications

Quality Level

200

Assay

≥92% (chromatography)
90.0-110.0% dry basis

form

solid

mol wt

Mr 5000-10000

technique(s)

transduction: suitable

shipped in

ambient

storage temp.

2-8°C

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General description

Protamine sulfate is a cationic polypeptide having a molecular weight of 5 kDa, isolated from salmon sperm.

Application

Protamine sulfate has been used in the transduction of
  • lentiviruses into human embryonic 293T cells
  • shRNA into mesenchymal stem cells/marrow stromal cells (MSC)
  • lenti Sca1-OCT4 plasmid into human cord blood hematopoietic progenitor CD34+ cells

Biochem/physiol Actions

Alkaline protamine binds to negatively charged heparin. Use of protamine during cardiovascular surgery sometime triggers anaphylactic reactions.
Protamine facilitates direct transfection of microbial cells, and of human cells using cationic liposomes. Protamine effectively neutralizes the anticoagulation effects of heparin.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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V Diness et al.
Thrombosis and haemostasis, 56(3), 318-322 (1986-12-15)
The neutralization of a low molecular weight heparin (LHN-1) and conventional heparin (CH) by protamine sulfate has been studied in vitro and in vivo. In vitro, the APTT activity of CH was completely neutralized in parallel with the anti-Xa activity.
shRNA-mediated decreases in c-Met levels affect the differentiation potential of human mesenchymal stem cells and reduce their capacity for tissue repair
Rosova I, et al.
Tissue Engineering: Part A, 16(8), 2627-2639 (2010)
Serious anaphylactic reactions due to protamine sulfate: a systematic literature review
Nybo M and Madsen JS
Basic and Clinical Pharmacology and Toxicology, 103(2), 192-196 (2008)
F L Sorgi et al.
Gene therapy, 4(9), 961-968 (1998-02-12)
A polycationic peptide, protamine sulfate, USP, has been shown to be able to condense plasmid DNA efficiently for delivery into several different types of cells in vitro by several different types of cationic liposomes. The monovalent cationic liposomal formulations (DC-Chol
Reprogramming of Human Cord Blood CD34+ Cells into Induced MSCs
Hematopoietic Differentiation of Human Pluripotent Stem Cells, 91-101 (2015)
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