P3369
Protamine sulfate
solid, 90.0-110.0% dry basis,≥92% (chromatography), suitable for transduction
Synonym(s):
Salmine sulfate from salmon sperm, Protamine sulfate
Product Name
Protamine sulfate, meets USP testing specifications
Agency
meets USP testing specifications
Quality Level
Assay
≥92% (chromatography)
90.0-110.0% dry basis
form
solid
mol wt
Mr 5000-10000
technique(s)
transduction: suitable
shipped in
ambient
storage temp.
2-8°C
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General description
Protamine sulfate is a cationic polypeptide having a molecular weight of 5 kDa, isolated from salmon sperm.
Application
Protamine sulfate has been used in the transduction of
- lentiviruses into human embryonic 293T cells
- shRNA into mesenchymal stem cells/marrow stromal cells (MSC)
- lenti Sca1-OCT4 plasmid into human cord blood hematopoietic progenitor CD34+ cells
Biochem/physiol Actions
Alkaline protamine binds to negatively charged heparin. Use of protamine during cardiovascular surgery sometime triggers anaphylactic reactions.
Protamine facilitates direct transfection of microbial cells, and of human cells using cationic liposomes. Protamine effectively neutralizes the anticoagulation effects of heparin.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
低风险生物材料
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Serious anaphylactic reactions due to protamine sulfate: a systematic literature review
Nybo M and Madsen JS
Basic and Clinical Pharmacology and Toxicology, 103(2), 192-196 (2008)
V Diness et al.
Thrombosis and haemostasis, 56(3), 318-322 (1986-12-15)
The neutralization of a low molecular weight heparin (LHN-1) and conventional heparin (CH) by protamine sulfate has been studied in vitro and in vivo. In vitro, the APTT activity of CH was completely neutralized in parallel with the anti-Xa activity.
F L Sorgi et al.
Gene therapy, 4(9), 961-968 (1998-02-12)
A polycationic peptide, protamine sulfate, USP, has been shown to be able to condense plasmid DNA efficiently for delivery into several different types of cells in vitro by several different types of cationic liposomes. The monovalent cationic liposomal formulations (DC-Chol
Reprogramming of Human Cord Blood CD34+ Cells into Induced MSCs
Hematopoietic Differentiation of Human Pluripotent Stem Cells, 91-101 (2015)
Meagan R Rollins et al.
Current protocols in immunology, 129(1), e97-e97 (2020-05-21)
T lymphocytes are capable of specific recognition and elimination of target cells. Physiological antigen recognition is mediated by the T cell receptor (TCR), which is an alpha beta heterodimer comprising the products of randomly rearranged V, D, and J genes.
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