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Merck
CN

P3476

Protein A–Agarose Fast Flow

50%, aqueous suspension

Synonym(s):

Protein A resin

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About This Item

UNSPSC Code:
41106500
NACRES:
NA.56
MDL number:
MFCD00165563
Form:
aqueous suspension
Biological source:
protein A from Staphylococcus aureus

Key Documents

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biological source

protein A from Staphylococcus aureus

description

protein A leaching <= 5 ng/mL

form

aqueous suspension

concentration

50%

matrix

6% Fast-flow beaded agarose

capacity

37-43 mg/mL binding capacity (IgG)

storage temp.

2-8°C

Application

Protein A-agarose is used for affinity chromatography, antibody purification, characterization, and protein A, G, and L resins. Protein A-agarose has been used to study the effects of protein A immunoadsorption in patients with chronic dilated cardiomyopathy as well as to study multiple sclerosis and gastric cancer.
Protein A–Agarose Fast Flow has been used:

  • in the immunoprecipitation of protein complex TRRAP/p400 from viral transfected human normal kidney and baby rat kidney cells
  • in the purification of polyclonal IgG from antisera
  • in the immunoprecipitation assay of human embryonic kidney cells transfected by HA-Ano9 plasmids

Features and Benefits

  • High capacity
  • Low leakage
  • Stable affinity medium for the purification of monoclonal and polyclonal antibodies
  • Ideal for rapid capture of immunoglobulins from cell culture

Physical form

Suspension containing 0.01% thimerosal

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
SLCK9517
SLCC7984
SLCC5921
SLCC3939
SLBZ5692

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Boris Gorbatyuk et al.
Molecular microbiology, 55(4), 1233-1245 (2005-02-03)
DnaA protein binds bacterial replication origins and it initiates chromosome replication. The Caulobacter crescentus DnaA also initiates chromosome replication and the C. crescentus response regulator CtrA represses chromosome replication. CtrA proteolysis by ClpXP helps restrict chromosome replication to the dividing
Verena K Hehle et al.
BMC biotechnology, 11, 128-128 (2012-01-03)
Interest in using plants for production of recombinant proteins such as monoclonal antibodies is growing, but proteolytic degradation, leading to a loss of functionality and complications in downstream purification, is still a serious problem. In this study, we investigated the
Henrik Buschmann et al.
Journal of cell science, 128(11), 2033-2046 (2015-04-25)
The preprophase band of microtubules performs the crucial function of marking the plane of cell division. Although the preprophase band depolymerises at the onset of mitosis, the division plane is 'memorized' by a cortical division zone to which the phragmoplast
Spiro Marangoudakis et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 32(30), 10365-10369 (2012-07-28)
Ca(V)2.2 (N-type) calcium channels control the entry of calcium into neurons to regulate essential functions but most notably presynaptic transmitter release. Ca(V)2.2 channel expression levels are precisely controlled, but we know little of the cellular mechanisms involved. The ubiquitin proteasome
Nolan J Hoffman et al.
Molecular metabolism, 41, 101048-101048 (2020-07-02)
Glycogen is a major energy reserve in liver and skeletal muscle. The master metabolic regulator AMP-activated protein kinase (AMPK) associates with glycogen via its regulatory β subunit carbohydrate-binding module (CBM). However, the physiological role of AMPK-glycogen binding in energy homeostasis
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