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Merck
CN

P3476

Protein A–Agarose Fast Flow

50%, aqueous suspension

Synonym(s):

Protein A resin

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About This Item

UNSPSC Code:
41106500
NACRES:
NA.56
MDL number:
Form:
aqueous suspension
Biological source:
protein A from Staphylococcus aureus
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biological source

protein A from Staphylococcus aureus

description

protein A leaching <= 5 ng/mL

form

aqueous suspension

concentration

50%

matrix

6% Fast-flow beaded agarose

capacity

37-43 mg/mL binding capacity (IgG)

storage temp.

2-8°C

Application

Protein A-agarose is used for affinity chromatography, antibody purification, characterization, and protein A, G, and L resins. Protein A-agarose has been used to study the effects of protein A immunoadsorption in patients with chronic dilated cardiomyopathy as well as to study multiple sclerosis and gastric cancer.
Protein A–Agarose Fast Flow has been used:

  • in the immunoprecipitation of protein complex TRRAP/p400 from viral transfected human normal kidney and baby rat kidney cells
  • in the purification of polyclonal IgG from antisera
  • in the immunoprecipitation assay of human embryonic kidney cells transfected by HA-Ano9 plasmids

Features and Benefits

  • High capacity
  • Low leakage
  • Stable affinity medium for the purification of monoclonal and polyclonal antibodies
  • Ideal for rapid capture of immunoglobulins from cell culture

Physical form

Suspension containing 0.01% thimerosal


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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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