P3738
Protein Kinase G Iβ human
≥95% (SDS-PAGE), recombinant, expressed in baculovirus infected Sf9 cells, buffered aqueous glycerol solution
Synonym(s):
cyclic-Guanosine Monophosphate-dependent Protein Kinase 1β human
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About This Item
Specific activity:
≥1.5 units/mg protein (20-fold stimulation by cGMP (5 μM))
Assay:
≥95% (SDS-PAGE)
Recombinant:
expressed in baculovirus infected Sf9 cells
recombinant
expressed in baculovirus infected Sf9 cells
Quality Segment
assay
≥95% (SDS-PAGE)
form
buffered aqueous glycerol solution
specific activity
≥1.5 units/mg protein (20-fold stimulation by cGMP (5 μM))
mol wt
76 kDa (monomer)
UniProt accession no.
relevant disease(s)
cancer
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... PRKG1(5592)
Application
Protein Kinase G is a serine/threonine-specific protein kinase that is activated by cGMP. Protein Kinase G Iβ is used to induce apoptosis and inhibit cell proliferation.
Biochem/physiol Actions
Protein Kinase G Iβ induces apoptosis in certain cell lines such as human breast cancer cell lines MCF-7 and MDA-MB-468. It inhibits cell proliferation and induces apoptosis in colon cancer cell lines.
Physical form
Solution in 20 mM Tris buffer, pH 7.4, 1 mM EDTA, 1 mM β-mercaptoethanol, 100 mM NaCl, 10 U/ml Trasylol, and 50% glycerol.
Other Notes
One unit will phosphorylate 1 micromole of VASPtide(RRKVSKQE) substrate per minute in 10 mM HEPES, pH 7.4, 5 mM MgCl2, 1 mM DTE and 0.2 mM EDTA.
Storage Class
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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Instructions
Global Trade Item Number
| SKU | GTIN |
|---|---|
| P3738-5UG | 04061838071859 |