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P3752

Phosphatase, Acid from potato

Name: Phosphatase, Acid from potato
Brand: SIGMA

lyophilized powder, ≥0.5 unit/mg solid

Synonym(s):

(Orthophosphoric-monoester phosphohydrolase acid optimum), Acid Phosphatase from potato

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About This Item

CAS Number:

9001-77-8

eCl@ss:

42010102

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-630-9

MDL number:

MFCD00131847

EC Number:

3.1.3.2 (BRENDA, IUBMB)

Specific activity:

≥0.5 unit/mg solid

Biological source:

potato

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biological source

potato

Quality Level

200

form

lyophilized powder

specific activity

≥0.5 unit/mg solid

mol wt

69 kDa

application(s)

diagnostic assay manufacturing

storage temp.

−20°C

SMILES string

O(CC(O)C)CC#C

InChI

1S/C6H10O2/c1-3-4-8-5-6(2)7/h1,6-7H,4-5H2,2H3

InChI key

GZCWLCBFPRFLKL-UHFFFAOYSA-N

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Application

Acid phosphatase is routinely used to dephosphorylate proteins and nucleic acids. Acid phosphatase is an acid hydrolase that normally resides in lysosomes. It is used as marker for the identification of lysosomes in subcellular fractionations. Acid phosphatase, product P3752, has been used as a control in order to study how Mycobacterium bovis BCG effects the levels of acid phosphatase activity in alveolar macrophages.

Biochem/physiol Actions

Acid phosphatase is a cytoplasmic lysosomal hydrolase that catalyzes the conversion of an orthophosphoric monoester and water to an alcohol and orthophosphate.

Other Notes

One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 4.8 at 37 °C.

pictograms

GHS08

signalword

Danger

hcodes

H334

pcodes

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

低风险生物材料

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An in vitro procedure for studying enzymic dephosphorylation of phytate in maize-soyabean feeds for turkey poults.

K Zyła et al.

The British journal of nutrition, 74(1), 3-17 (1995-07-01)

An in vitro method was developed to predict inorganic P release from maize-soyabean poultry feeds containing supplemental phytase (EC 3.1.3.8), and to quantify the effect of acid phosphatase (EC 3.1.3.2), fungal protease (EC 3.4.23.6) and Aspergillus niger cellulase (EC 3.2.1.4)

Detecting acid phosphatase enzymatic activity with phenol as a chemical exchange saturation transfer magnetic resonance imaging contrast agent (PhenolCEST MRI).

Jia Zhang et al.

Biosensors & bioelectronics, 141, 111442-111442 (2019-06-30)

Phenol contains an exchangeable hydroxyl proton resonant at 4.8 ppm from the resonance frequency of water in the 1H nuclear magnetic resonance (1H NMR) spectrum, enabling itself to be detected at sub-mM concentration by either chemical exchange saturation transfer magnetic resonance

Reconstitution of Vanadium Haloperoxidase's Catalytic Activity by Boric Acid-Towards a Potential Biocatalytic Role of Boron.

Filipe Natalio et al.

Chemistry (Weinheim an der Bergstrasse, Germany), 23(21), 4973-4980 (2017-03-02)

Boron's unusual properties inspired major advances in chemistry. In nature, the existence and importance of boron has been fairly explored (e.g. bacterial signaling, plant development) but its role as biological catalyst was never reported. Here, we show that boric acid

Enhanced recovery of murine alveolar macrophages: morphological and functional characteristics following intravenous injection of heat-killed Mycobacterium bovis BCG.

I N Mbawuike et al.

Infection and immunity, 51(2), 483-489 (1986-02-01)

The kinetics of induction of the bronchoalveolar cell population (i.e., alveolar macrophages [AM], lymphocytes, and polymorphonuclear leukocytes) was studied in mice inoculated intravenously with heat-killed Mycobacterium bovis BCG. Injection of BCG at 100 and 500 micrograms but not at 10

Relative quantification of peptide phosphorylation in a complex mixture using 18O labeling.

Julia R Smith et al.

Physiological genomics, 31(2), 357-363 (2007-08-09)

We have developed a method to determine the degree of phosphorylation of a peptide in a complex mixture without enrichment or operation of the mass spectrometer in negative ion mode. Yeast lysate containing known amounts of synthetic peptides (VPQLEIVPNSAEERLHSMK and

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Protocols

Enzymatic Assay of Acid Phosphatase (EC 3.1.3.2)

酸性磷酸酶的酶促测定（EC 3.1.3.2）

在测定酸性磷酸酶活性时，本操作流程使用对硝基苯基磷酸盐进行比色法测定。酶活性是使用分光光度计在410 nm处测定的。

Global Trade Item Number

SKU	GTIN
P3752-1G	04061835506552
P3752-100MG	04061834372837

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