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P4039

Phospholipase C Peptide

Name: Phospholipase C Peptide
Brand: SIGMA

lyophilized powder, Type XIV, ≥150 units/mg protein

Synonym(s):

Lecithinase C, Lipophosphodiesterase I, PC-PLC, Phosphatidylcholine cholinephosphohydrolase

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About This Item

CAS Number:

9001-86-9

UNSPSC Code:

41106300

NACRES:

NA.32

EC Number:

232-638-2

MDL number:

MFCD00131895

EC Number:

3.1.4.3 (BRENDA, IUBMB)

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Product Name

Phospholipase C from Clostridium perfringens (C. welchii), Type XIV, lyophilized powder, ≥150 units/mg protein

biological source

bacterial (Clostridium perfringens)

Quality Level

200

type

Type XIV

form

lyophilized powder

specific activity

≥150 units/mg protein

greener alternative product characteristics

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

greener alternative category

Enabling,

storage temp.

−20°C

Gene Information

Clostridium perfringens str. 13 ... plc(988262)

General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in biodiesel research. For more information see the article in biofiles.

Application

Phospholipase C from Clostridium perfringens has been used as a positive control in sandwich enzyme linked immunosorbent assay (ELISA) and ELISA.

Biochem/physiol Actions

Hydrolyzes the phosphate bond on phosphatidylcholine and other glycerophospholipids yielding diacylglycerol; this enzyme will also hydrolyze the phosphate bonds of sphingomyelin, cardiolipin, choline plasmalogen and ceramide phospholipids.

Physical form

Lyophilized powder in buffered salts

Preparation Note

Chromatographically purified

Other Notes

One unit will liberate 1.0 μmole of water soluble organic phosphorus from egg yolk L-α-phosphatidylcholine per min at pH 7.3 at 37 °C.

pictograms

GHS08

signalword

Danger

hcodes

H334

pcodes

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

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Development and evaluation of indirect enzyme-linked immunosorbent assays for the determination of immune response to multiple clostridial antigens in vaccinated captive bred southern white rhinoceros (Ceratotherium simum simum).

Angela Buys et al.

Acta veterinaria Scandinavica, 62(1), 57-57 (2020-10-09)

An overall increase in poaching of white rhinoceros results in captive breeding becoming a significant component of white rhinoceros conservation. However, this type of conservation comes with its own difficulties. When wildlife is captured, transported and/or confined to a boma

In vitro inhibitory effect of hen egg white lysozyme on Clostridium perfringens type A associated with broiler necrotic enteritis and its alpha-toxin production.

G Zhang et al.

Letters in applied microbiology, 42(2), 138-143 (2006-01-31)

Clostridium perfringens type A causes both clinical and subclinical forms of necrotic enteritis in domestic avian species. In this study the inhibitory effect of hen egg white lysozyme on the vegetative form of Cl. perfringens type A and the production

Sandwich ELISA detection of Clostridium perfringens cells and alpha-toxin from field cases of necrotic enteritis of poultry

McCourt MT, et al.

Veterinary Microbiology, 106(3-4), 259-264 (2005)

In vitro inhibitory effect of hen egg white lysozyme on Clostridium perfringens type A associated with broiler necrotic enteritis and its alpha-toxin production

Zhang G, et al.

Letters in Applied Microbiology, 42(2), 138-143 (2006)

A measured approach: determining the PLCγ1 docking site on Itk using a biochemical ruler.

Ranajeet Ghose

Journal of molecular biology, 425(4), 679-682 (2012-12-26)

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Global Trade Item Number

SKU	GTIN
P4039-125UN	04061833621745

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