P4252
Phosphatase, Alkaline from Escherichia coli
ammonium sulfate suspension, 30-90 units/mg protein (modified Warburg-Christian, in glycine buffer)
Synonym(s):
Orthophosphoric-monoester phosphohydrolase (alkaline optimum)
biological source
Escherichia coli
Quality Level
form
ammonium sulfate suspension
specific activity
30-90 units/mg protein (modified Warburg-Christian, in glycine buffer)
storage temp.
2-8°C
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Application
Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It may be used for protein labeling when high sensitivity is required. Product P4252 has been used for bulk digestion of tRNA . Product P4252 is provided in the ratio of 30-90 units/mg of protein.
Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It may be used for protein labeling when high sensitivity is required. Product P4252 is an ammonium sulfate suspension in glycine buffer.
Biochem/physiol Actions
Alkaline phosphatase, from Escherichia coli, is a dimeric, non-glycosylated protein which mainly reside in the periplasmic space. Three known isoforms exist. The enzyme requires zinc, and is activated by magnesium. E. coli akaline phosphatase has a broad specificity for phosphate esters.
Alkaline phosphatase, from Escherichia coli, is a dimeric, non-glycosylated protein which mainly reside in the periplasmic space. Three known isoforms exist. The enzyme requires zinc, and is activated by magnesium. E. coli akaline phosphatase has a broad specificity for phosphate esters.
Physical form
Suspension in 2.5 M (NH4)2SO4
Analysis Note
The activity at pH 8.0 at 25 °C is approximately the same as at pH 10.4 at 37 °C.
Other Notes
One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 10.4 at 37 °C.
Disclaimer
Presence of 0.1% SDS in the alkaline phosphatase assays will inhibit any nuclease contamination in the sample.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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E. coli Alkaline Phosphatase
1.Reid, T., and Wilson
The Enzymes, 4, 373-373 (1971)
The universal YrdC/Sua5 family is required for the formation of threonylcarbamoyladenosine in tRNA
Basma El Yacoubi, Benjamin Lyons, et al.
RNA, 37, 2894-2909 (2009)
Tamaha Yamaguchi et al.
Frontiers in microbiology, 11, 570081-570081 (2021-02-09)
The labile fraction of dissolved organic phosphorus (DOP) - predominantly consisting of phosphoric esters - is an important microbial P source in the subtropical oligotrophic ocean. However, unlike phosphate, knowledge for labile DOP is still limited due to the scarcity
Natalia Krawczun et al.
MethodsX, 8, 101340-101340 (2021-08-26)
Genes encoding proteins 'toxic' to recombinant host are difficult for cloning/expression and recombinant clones are unstable. Even tightly controlled inducible T7-lac, PBAD, PL, PR promoters are not totally silent in an uninduced state and thus not adequate for highly toxic
R A Anderson et al.
Proceedings of the National Academy of Sciences of the United States of America, 72(1), 394-397 (1975-01-01)
To facilitate the study of individual metal binding sites of polymeric metalloproteins, conversion of exchange-labile Co(II) in E. coli alkaline phosphatase (EC 3.1.3.1) to exchange-inert Co(III) was examined. Oxidation of Co(II) alkaline phosphatase with hydrogen peroxide results in a single
Articles
Instructions for working with enzymes supplied as ammonium sulfate suspensions
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