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P4399

β-Lactamase from Enterobacter cloacae

Type III, lyophilized powder, 6-18 units/mg protein (using benzylpenicillin)

Synonym(s):

β-Lactamase I, β-Lactamase II, Cephalosporinase, Penicillin amido-β-lactam hydrolase, Penicillinase from Enterobacter cloacae

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About This Item

CAS Number:
9073-60-3
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
3.5.2.6 (BRENDA, IUBMB)
MDL number:
MFCD00131819
Specific activity:
6-18 units/mg protein (using benzylpenicillin)
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type

Type III

form

lyophilized powder

specific activity

6-18 units/mg protein (using benzylpenicillin)

composition

Protein, ~10%

storage temp.

2-8°C

Application

β--lactamase is used to inactivate β-lactam antibiotics by breaking open the β-lactam ring. β--lactamase is used to study antibiotic resistance and resistance suppression. Product P4399 is produced from Enterobacter cloacae.

Biochem/physiol Actions

β--lactamase inactivates β-lactam antibiotics by breaking open the β-lactam ring.

Physical form

Lyophilized powder containing sodium phosphate and sodium citrate buffer salts

Preparation Note

Chromatographically purified

Analysis Note

Protein determined by biuret

Other Notes

One unit will hydrolyze 1.0 μmole of benzylpenicillin per min at pH 7.0 at 25 °C. This International Unit (using benzylpenicillin as substrate) is approximately equal to 600 Levy or 75 Pollock units.

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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