P4399
β-Lactamase from Enterobacter cloacae
Type III, lyophilized powder, 6-18 units/mg protein (using benzylpenicillin)
Synonym(s):
β-Lactamase I, β-Lactamase II, Cephalosporinase, Penicillin amido-β-lactam hydrolase, Penicillinase from Enterobacter cloacae
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
6-18 units/mg protein (using benzylpenicillin)
type
Type III
form
lyophilized powder
specific activity
6-18 units/mg protein (using benzylpenicillin)
composition
Protein, ~10%
storage temp.
2-8°C
Application
β--lactamase is used to inactivate β-lactam antibiotics by breaking open the β-lactam ring. β--lactamase is used to study antibiotic resistance and resistance suppression. Product P4399 is produced from Enterobacter cloacae.
Biochem/physiol Actions
β--lactamase inactivates β-lactam antibiotics by breaking open the β-lactam ring.
Physical form
Lyophilized powder containing sodium phosphate and sodium citrate buffer salts
Preparation Note
Chromatographically purified
Analysis Note
Protein determined by biuret
Other Notes
One unit will hydrolyze 1.0 μmole of benzylpenicillin per min at pH 7.0 at 25 °C. This International Unit (using benzylpenicillin as substrate) is approximately equal to 600 Levy or 75 Pollock units.
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signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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