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Merck
CN

P4553

6-Phosphogluconic Dehydrogenase from yeast

lyophilized powder, ≥3.0 units/mg solid

Synonym(s):

6-Phospho-D-gluconate:NADP+ 2-oxidoreductase (decarboxylating), 6-Phosphogluconic Dehydrogenase from yeast

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About This Item

CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
MDL number:
Specific activity:
≥3.0 units/mg solid
Biological source:
yeast
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Quality Segment

biological source

yeast

form

lyophilized powder

specific activity

≥3.0 units/mg solid

mol wt

52 kDa

storage temp.

2-8°C

General description

6-Phosphogluconic Dehydrogenase from yeast comprises of a NADP+ binding domain, α helical hydrophobic interaction domain and a C-terminal domain. It exists as dimer and the C-terminal region is crucial for the substrate binding and release.

Application

6-Phosphogluconic Dehydrogenase from yeast has been used in hexokinase (HK) and xylose reductase (XR) activities in cell lysate.

Biochem/physiol Actions

Enzyme activity is determined by measuring the reduction of NAD+ or NADP+. High levels of NADPH are believed to inhibit the enzyme, while 6-phosphogluconate acts to activate the enzyme.

Other Notes

One unit will oxidize 1.0 μmole of 6-phospho-D-gluconate to D-ribulose 5-phosphate and CO2 per min at pH 7.4 at 37 °C in the presence of NADP+.


pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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