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P4716

Sigma-Aldrich

Pectinase from Aspergillus niger

greener alternative

BioReagent, suitable for plant cell culture, aqueous glycerol solution, ≥5 units/mg protein (Lowry)

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Synonym(s):
Poly-(1,4-α-D-galacturonide) glycanohydrolase, Polygalacturonase solution from Aspergillus niger
CAS Number:
9032-75-1
Enzyme Commission number:
3.2.1.15 (BRENDA, IUBMB)
EC Number:
232-885-6
MDL number:
MFCD00131809
NACRES:
NA.54

biological source

Aspergillus niger

Quality Level

200

product line

BioReagent

form

aqueous glycerol solution

specific activity

≥5 units/mg protein (Lowry)

greener alternative product characteristics

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

technique(s)

cell culture | plant: suitable

greener alternative category

, Enabling

storage temp.

2-8°C

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General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in cellulosic ethanol research. For more information see the article in biofiles and Enzymes for Alternative Energy Research.

Application

Petctinase from Aspergillus niger has been used:
  • for protoplasts liberation
  • in the protoplast enzyme mixture for enzyme digestion
  • to conduct partial saccharification of sugars
  • to study their role in the invasion of plant tissues by phytopathogens, the spoilage of produce and various food processing and plant biotechnology applications

Used in plant protoplast preparation to digest cell wall prior to organelle isolation.

Biochem/physiol Actions

Pectolytic enzyme preparation produced from a selected strain of Aspergillus niger: contains mainly pectintranseliminase, polygalacturonase, and pectinesterase and small amounts of hemicellulases and cellulases. Pectinases hydrolyses pectin, which is a component of the cell wall. They may attack methyl-esterified pectin or de-esterified pectin. It is a source of pectinase activity, also containing cellulase and hemicellulase activities.Pectinase catalyzes the random hydrolysis of a-(1-4)-Dgalactosiduronic linkages in pectin and other galacturonans.

Unit Definition

One unit will liberate 1.0 μmole of galacturonic acid from polygalacturonic acid per min at pH 4.0 at 25 °C.

Physical form

Solution in 40% glycerol

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Hazard Classifications

Resp. Sens. 1

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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Tolerance mechanisms in mercury-exposed Chromolaena odorata (lf) RM King et H. Robinson, a potential phytoremediator
Alcantara HJP, et al.
Journal of Degraded and Mining Lands Management, 1(1), 09-20 (2013)
Tony Heitkam et al.
The Plant journal : for cell and molecular biology, 103(1), 32-52 (2020-01-26)
If two related plant species hybridize, their genomes may be combined and duplicated within a single nucleus, thereby forming an allotetraploid. How the emerging plant balances two co-evolved genomes is still a matter of ongoing research. Here, we focus on
Karyotypes of some species of Castanopsis, Lithocarpus and Quercus (Fagaceae) from Khun Mae Kuang Forest in Chiang Mai province, northern Thailand
Chokchaichamnankit P, et al.
Thai Forest Bulletin , 38-44 (2007)
Eva Wegel
Methods in molecular biology (Clifton, N.J.), 1536, 3-21 (2017-01-31)
This chapter describes methods to detect gene loci or gene transcripts by fluorescence labeling. Fluorescence in situ hybridization (FISH) can be used to identify the positions of genes or BACs or the distribution of repetitive sequences on metaphase chromosomes as
Nicola Schmidt et al.
Annals of botany, 128(3), 281-299 (2021-03-18)
Endogenous pararetroviruses (EPRVs) are widespread components of plant genomes that originated from episomal DNA viruses of the Caulimoviridae family. Due to fragmentation and rearrangements, most EPRVs have lost their ability to replicate through reverse transcription and to initiate viral infection.
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Protocols

Enzymatic Assay of Pectinase

To measure pectinase activity, a titrimetric stop reaction assay is used. One unit of pectinase will liberate 1 μmol of galacturonic acid from poly-galacturonic acid per hour at pH 4.0 at 25 °C.

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