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P4716

Pectinase from Aspergillus niger

Name: Pectinase from <I>Aspergillus niger</I>
Brand: SIGMA

BioReagent, suitable for plant cell culture, aqueous glycerol solution, ≥5 units/mg protein (Lowry)

Synonym(s):

Poly-(1,4-α-D-galacturonide) glycanohydrolase, Polygalacturonase solution from Aspergillus niger

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About This Item

CAS Number:

9032-75-1

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-885-6

MDL number:

MFCD00131809

EC Number:

3.2.1.15 (BRENDA, IUBMB)

Specific activity:

≥5 units/mg protein (Lowry)

Biological source:

Aspergillus niger

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biological source

Aspergillus niger

Quality Level

200

product line

BioReagent

form

aqueous glycerol solution

specific activity

≥5 units/mg protein (Lowry)

greener alternative product characteristics

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

technique(s)

cell culture | plant: suitable

greener alternative category

, Enabling

storage temp.

2-8°C

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Related Categories

Cell Culture & Analysis

Cell Culture Media & Buffers

Cell Culture Supplements & Reagents

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General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in cellulosic ethanol research. For more information see the article in biofiles and Enzymes for Alternative Energy Research.

Application

Petctinase from Aspergillus niger has been used:

for protoplasts liberation
in the protoplast enzyme mixture for enzyme digestion
to conduct partial saccharification of sugars
to study their role in the invasion of plant tissues by phytopathogens, the spoilage of produce and various food processing and plant biotechnology applications

Used in plant protoplast preparation to digest cell wall prior to organelle isolation.

Biochem/physiol Actions

Pectolytic enzyme preparation produced from a selected strain of Aspergillus niger: contains mainly pectintranseliminase, polygalacturonase, and pectinesterase and small amounts of hemicellulases and cellulases. Pectinases hydrolyses pectin, which is a component of the cell wall. They may attack methyl-esterified pectin or de-esterified pectin. It is a source of pectinase activity, also containing cellulase and hemicellulase activities.Pectinase catalyzes the random hydrolysis of a-(1-4)-Dgalactosiduronic linkages in pectin and other galacturonans.

Physical form

Solution in 40% glycerol

Other Notes

One unit will liberate 1.0 μmole of galacturonic acid from polygalacturonic acid per min at pH 4.0 at 25 °C.

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

pictograms

GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

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Karyotypes of some species of Castanopsis, Lithocarpus and Quercus (Fagaceae) from Khun Mae Kuang Forest in Chiang Mai province, northern Thailand

Chokchaichamnankit P, et al.

Thai Forest Bulletin , 38-44 (2007)

Satellite DNA landscapes after allotetraploidization of quinoa (Chenopodium quinoa) reveal unique A and B subgenomes.

Tony Heitkam et al.

The Plant journal : for cell and molecular biology, 103(1), 32-52 (2020-01-26)

If two related plant species hybridize, their genomes may be combined and duplicated within a single nucleus, thereby forming an allotetraploid. How the emerging plant balances two co-evolved genomes is still a matter of ongoing research. Here, we focus on

Foliar Essential Oil Glands of Eucalyptus Subgenus Eucalyptus (Myrtaceae) Are a Rich Source of Flavonoids and Related Non-Volatile Constituents.

Jason Q D Goodger et al.

PloS one, 11(3), e0151432-e0151432 (2016-03-16)

The sub-dermal secretory cavities (glands) embedded within the leaves of Eucalyptus (Myrtaceae) were once thought to be the exclusive repositories of monoterpene and sesquiterpene oils. Recent research has debunked this theory and shown that abundant non-volatile compounds also occur within

Tolerance mechanisms in mercury-exposed Chromolaena odorata (lf) RM King et H. Robinson, a potential phytoremediator

Alcantara HJP, et al.

Journal of Degraded and Mining Lands Management, 1(1), 09-20 (2013)

Preparation and Fluorescent Analysis of Plant Metaphase Chromosomes.

Trude Schwarzacher

Methods in molecular biology (Clifton, N.J.), 1370, 87-103 (2015-12-15)

Good preparations are essential for informative analysis of both somatic and meiotic chromosomes, cytogenetics, and cell divisions. Fluorescent chromosome staining allows even small chromosomes to be visualized and counted, showing their morphology. Aneuploidies and polyploidies can be established for species

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Protocols

Enzymatic Assay of Pectinase

To measure pectinase activity, a titrimetric stop reaction assay is used. One unit of pectinase will liberate 1 μmol of galacturonic acid from poly-galacturonic acid per hour at pH 4.0 at 25 °C.

果胶酶的酶促检测

在测定果胶酶活性时，使用了一种滴定终止反应法进行测定。在25 °C、pH 4.0条件下，一个单位的果胶酶每小时可从多聚半乳糖醛酸释放1 μmol 的半乳糖醛酸。

Global Trade Item Number

SKU	GTIN
P4716-100KU	04061834375111
P4716-25KU	04061834375128
P4716-5KU	04061838256171
P4716-10KU	04061838256164

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