P4937
Percoll®
pH 8.5-9.5 (25 °C), suitable for cell culture
Synonym(s):
Density gradient medium
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About This Item
sterility
aseptically filled
Quality Level
form
colloidal
technique(s)
cell culture | mammalian: suitable, single cell analysis: suitable
pH
8.5-9.5 (25 °C)
density
1.13 g/mL±0.005 g/mL at 25 °C (lit.)
storage temp.
2-8°C
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General description
Percoll® is a classic medium for density gradient centrifugation of cells, viruses, and subcellular particles. It consists of colloidal silica particles of 15-30 nm diameter (23% w/w in water), which have been coated with polyvinylpyrrolidone (PVP). The PVP coating renders the product completely non-toxic and ideal for use with biological materials. Due to its heterogeneity in particle size, sedimentation occurs at different rates, spontaneously creating very smooth, isometric gradients in the range of 1.0-1.3 g/ml.
Application
Percoll consists of colloidal silica particles of 15-30 nm diameter (23% w/w in water) which have been coated with polyvinylpyrrolidone (PVP). It is used in cell separation and organelle isolation.
Percoll consists of colloidal silica particles of 15-30 nm diameter (23% w/w in water) which have been coated with polyvinylpyrrolidone (PVP). It is used to establish non-toxic low viscosity, low osmolarity density gradients that can be used to isolate cells, organelles and viruses. It has been used to separate inner and outer membrane vesicles from E. coli. Percoll is isosmotic throughout the gradient, non-toxic, and is easily removed from the purified materials.
Percoll® has been used in the centrifugal separation of:
- neonatal rat ventricular cardiomyocytes
- leucocytes from a blood sample
- peritubular myoid (PM) cells
Features and Benefits
- Low osmolality permits precise adjustment to physiological conditions without significant interference from the medium.
- Compatible with living cells and viruses and allows separation and recovery of intact, fully active systems.
- Impermeable to biological membranes, resulting in no change of buoyant density of particles during centrifugation.
- Spontaneous formation of gradient during centrifugation allows mixing of large sample volumes in the centrifuge tubes.
- Low viscosity results in rapid formation of gradients and particle separation.
- Most biological particles having sedimentation coefficient values greater than 60S can be successfully isolated in Percoll gradients.
Legal Information
Percoll is a registered trademark of Cytiva
Storage Class
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
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Hila Doron et al.
Cell reports, 28(7), 1785-1798 (2019-08-15)
Melanoma is the deadliest skin cancer due to its high rate of metastasis, frequently to the brain. Brain metastases are incurable; therefore, understanding melanoma brain metastasis is of great clinical importance. We used a mouse model of spontaneous melanoma brain
Density gradients prepared from colloidal silica particles coated by polyvinylpyrrolidone (Percoll).
H Pertoft et al.
Analytical biochemistry, 88(1), 271-282 (1978-07-15)
Maryam Ghaedi et al.
The Journal of experimental medicine, 217(3) (2019-12-10)
Lung group 2 innate lymphoid cells (ILC2s) drive allergic inflammation and promote tissue repair. ILC2 development is dependent on the transcription factor retinoic acid receptor-related orphan receptor (RORα), which is also expressed in common ILC progenitors. To elucidate the developmental
Ann-Chang Cheng et al.
Veterinary immunology and immunopathology, 121(3-4), 206-215 (2007-11-06)
Brown-marbled grouper Epinephelus fuscoguttatus which had been fed diets containing sodium alginate and kappa (kappa)-carrageenan at 5, 10 and 20gkg(-1), respectively after 0, 2, 4, 6, 8 and 14 weeks were examined for survival, growth, innate cellular and humoral responses
Nunzia Pastore et al.
Nature communications, 11(1), 2461-2461 (2020-05-20)
It is well established that pluripotent stem cells in fetal and postnatal liver (LPCs) can differentiate into both hepatocytes and cholangiocytes. However, the signaling pathways implicated in the differentiation of LPCs are still incompletely understood. Transcription Factor EB (TFEB), a
Protocols
To prepare a Percoll gradient, the osmolality of Percoll must be adjusted with saline or cell culture medium to make Percoll isotonic with physiological salt solutions.
为了配置Percoll梯度液，必须先用生理盐水或细胞培养基将Percoll调至生理性渗透压。
Related Content
Global Trade Item Number
| SKU | GTIN |
|---|---|
| P4937-100ML | 04061837036606 |
| P4937-500ML | 04061837036644 |
| P4937-25ML | 04061837036620 |
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