P5208
Platelet-Derived Endothelial Cell Growth Factor human
≥97% (SDS-PAGE), recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder, suitable for cell culture
Synonym(s):
PD-ECGF
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About This Item
Biological source:
human
Recombinant:
expressed in baculovirus infected Sf21 cells
Assay:
≥97% (SDS-PAGE)
Form:
lyophilized powder
Mol wt:
49 kDa
Impurities:
<1 EU/mg
Product Name
Platelet-Derived Endothelial Cell Growth Factor human, recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder, suitable for cell culture
biological source
human
Quality Segment
recombinant
expressed in baculovirus infected Sf21 cells
assay
≥97% (SDS-PAGE)
form
lyophilized powder
potency
5-30 ng/mL EC50
mol wt
49 kDa
packaging
pkg of 10 μg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
impurities
<1 EU/mg
UniProt accession no.
storage temp.
−20°C
Gene Information
human ... PDGFA(100126841), TYMP(1890)
General description
Platelet-Derived Endothelial Cell Growth Factor (PD-ECGF) is an endothelial cell mitogen which was originally purified from human platelets. In contrast with the endothelial mitogens of the fibroblast growth factor (FGF) family, PD-ECGF is an endothelial cell growth factor initially purified from human platelets, which does not bind to heparin and does not stimulate the proliferation of fibroblasts. This is in contrast with the effect of PDGF, which stimulates the growth of human foreskin fibroblasts, but is inactive on endothelial cells. PD-ECGF will stimulate endothelial cells in vitro and in vivo. PD-ECGF is chemotactic for bovine aortic endothelial cells. PD-ECGF does not induce smooth muscle cell migration. PD-ECGF is involved in angiogenesis and has potent angiogenic activity both in the developing vascular system of the chick chorioallantoic membrane and in vascularization of tumors in nude mice. PD-ECGF has a pI of 4.0-4.8 and a molecular weight of 49 kDa.
The biological activity of recombinant, human PD-ECGF is measured in a cell proliferation assay using human umbilical vein endothelial cells. The EC50 is defined as the effective concentration of growth factor that elicits a 50% increase in cell growth in a cell based bioassay
The biological activity of recombinant, human PD-ECGF is measured in a cell proliferation assay using human umbilical vein endothelial cells. The EC50 is defined as the effective concentration of growth factor that elicits a 50% increase in cell growth in a cell based bioassay
Application
PD-ECGF is involved in angiogenesis and has potent angiogenic activity both in the developing vascular system of the chick chorioallantoic membrane and in vascularization of tumors in nude mice.
Biochem/physiol Actions
Platelet-derived endothelial cell growth factor is a 45 kDa endothelial cell mitogen of pI 4.0-4.8 originally purified from human platelets. PD-ECGF is chemotactic for bovine aortic endothelial cells, however it does not induce smooth muscle cell migration. PD-ECGF is involved in angiogenesis and has potent angiogenic activity both in the developing vascular system of the chick chorioalantoic membrane and vascularization of tumor cells in nude mice. PD-ECGF is highly expressed in some tumors.
Physical form
Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.5 mg bovine serum albumin.
Analysis Note
The proliferative activity of human PD-ECGF is measured in culture by using human umbilical vein endothelial cells.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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