P5267
L-Proline p-nitroanilide trifluoroacetate salt
≥99% (TLC), suitable for ligand binding assays
Synonym(s):
N-(4-Nitrophenyl)pyrrolidine-2-carboxamide, P-pNA, Pro-pNA
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About This Item
Empirical Formula (Hill Notation):
C11H13N3O3 · C2HF3O2
CAS Number:
Molecular Weight:
349.26
UNSPSC Code:
12352209
eCl@ss:
32160406
PubChem Substance ID:
NACRES:
NA.26
MDL number:
Product Name
L-Proline p-nitroanilide trifluoroacetate salt, prolyl aminopeptidase substrate
Quality Level
assay
≥99% (TLC)
form
powder
technique(s)
ligand binding assay: suitable
color
white to yellow
storage temp.
2-8°C
SMILES string
OC(=O)C(F)(F)F.[O-][N+](=O)c1ccc(NC(=O)[C@@H]2CCCN2)cc1
InChI
1S/C11H13N3O3.C2HF3O2/c15-11(10-2-1-7-12-10)13-8-3-5-9(6-4-8)14(16)17;3-2(4,5)1(6)7/h3-6,10,12H,1-2,7H2,(H,13,15);(H,6,7)/t10-;/m0./s1
InChI key
KYRVEVYREUUAKH-PPHPATTJSA-N
General description
Proline p-nitroanilide (P-pNA) is a colorimetric substrate for prolyl aminopeptidase (proline iminopeptidase), an enzyme that releases proline from the N-terminus of small peptides.
Application
L-Proline p-nitroanilide trifluoroacetate salt has also been used as a monopeptide substrate for measuring the amidolytic activity of fibrillated peptide catalyst, PC4.
Proline p-nitroanilide (P-pNA) has been used as a substrate for prolyl aminopeptidase (proline iminopeptidase) from cabbage leaves.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Margarita Marinova et al.
Protein and peptide letters, 16(2), 207-212 (2009-02-10)
Chick-pea (Cicer arietinum L.) cotyledons are unique source of aminopeptidase - 8-9 U/g cotyledons was observed using L-leucine-p-nitroanilide as substrate. The aminopeptidase was purified (65 kDa, pI 4.8 ) reaching a specific activity of 220 U/mg at pH 7.0-7.2 and
Hongyu Yang et al.
World journal of microbiology & biotechnology, 32(11), 176-176 (2016-09-16)
Prolyl aminopeptidases are specific exopeptidases that catalyze the hydrolysis of the N-terminus proline residue of peptides and proteins. In the present study, the prolyl aminopeptidase gene (pap) from Aspergillus oryzae JN-412 was optimized through the codon usage of Pichia pastoris.
Paul J Lijnen et al.
Journal of the renin-angiotensin-aldosterone system : JRAAS, 6(2), 69-77 (2006-02-14)
To determine whether the aminopeptidase B inhibitor, arphamenine A, could affect collagen production and expression in control and TGF-ss1-treated cardiac fibroblasts. Cardiac fibroblasts from passage 2 from normal male adult rats were cultured to confluency and incubated with and without
Global Trade Item Number
| SKU | GTIN |
|---|---|
| P5267-1G | 04061834377221 |
| P5267-2G | 04061834377320 |