P5282
Phalloidin, Fluorescein Isothiocyanate Labeled
sequence Amanita phalloides(synthetic: peptide sequence)
biological source
sequence from Amanita phalloides (synthetic: peptide sequence)
Quality Level
form
solid
fluorescence
λex 495 nm; λem 520 nm(lit.)
storage temp.
−20°C
Related Categories
General description
Application
- To visualize F-Actin reorganization in primary neonatal cardiomyocytes (PNCMs) and H9C2 cells (rat cardiac myoblasts) following endothelin-1 (ET-1) and angiotensin II (Ang II) treatment.
- In immunochemistry to label microfilament.
- In immunofluorescence analysis to stain F-actin.
Biochem/physiol Actions
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 2 Dermal - Acute Tox. 2 Inhalation - Acute Tox. 2 Oral
Storage Class Code
6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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705578-5MG-PW
PL860-CGA/SHF-1EA
MMYOMAG-74K-13
1000309185
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If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
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The lot specific COA document can be found by entering the lot number above under the "Documents" section.
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What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
How do I dissolve Product P5282, Phalloidin, Fluorescein Isothiocyanate Labeled?
This product is soluble in methanol, ethanol, butanol and pyridine. It is soluble in cold water at a concentration of 5 mg/mL; it is much more soluble in hot water. See: The chemicals encyclopedia published by the Royal Society of Chemistry, 12th ed., entry# 7336 (1996). Solutions should be prepared fresh, and protected from light whenever possible.
How do I prepare stock solutions of Product P5282, Phalloidin, Fluorescein Isothiocyanate Labeled?
Stock solutions of phalloidin conjugates have been made in methanol or DMSO at 0.1 to 5 mg/mL. Make final dilutions in aqueous physiological buffers for a staining range from 0.1 μM to 100 μM with corresponding incubation times of 15 minutes to 72 hours.
What are the excitation and emission wavelengths used for fluorescent detection of Product P5282, Phalloidin, Fluorescein Isothiocyanate Labeled?
Excitation wavelengths of 540-545 nm and emission wavelengths of 570-573 nm can be used. See: Faulstich, H., J. Muscle Res. Cell Motility, 9, 370 (1988), and Waggoner, A. et al., Methods in Cell Biology, 30, 449 (1989).
How do I stain cells with Product P5282, Phalloidin, Fluorescein Isothiocyanate Labeled?
A typical application for staining cells can be found in the product information sheet (under Documents, above).
How do I store Product P5282, Phalloidin, Fluorescein Isothiocyanate Labeled?
The product should be stored at a powder in the freezer at -20°C.
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