Anti-Par-4 (Prostate Apoptosis Response-4) antibody produced in rabbit

PRKC apoptosis WT1 regulator protein (PAWR) is also known as prostate apoptosis response 4 protein (Par-4). The PAWR gene is mapped to human chromosome 12q21.2. It is a 40 kDa protein expressed ubiquitously in mammals. It was first identified in prostate tumors.It has a leucine zipper domain and nuclear localization sequences. A core domain comprising of region 137-195 amino acids is critical for the apoptotic function of PAR-4. It interacts and modulates the activity of protein kinase C.

synthetic peptide corresponding to the C-terminus of human Par-4 (amino acids 324-342) conjugated to KLH.

Anti-Par-4 (Prostate Apoptosis Response-4) antibody produced in rabbit may be used in immunoblotting.

Par-4 protein interacts with Wilm′s tumor suppressor WT1 and modulates its transcription and growth suppression function. The gene for Par-4 is down-regulated by oncogenic Ras via the Raf-1- mitogen-activated protein kinase/extracellular signal-regulated kinase (MEK-ERK) pathway in immortalized fibroblasts. Overexpression of the PAR4 gene causes down-regulation of B-cell lymphoma-2 (Bcl-2) in fibroblasts and prostate cancer cells. Par-4 protein plays an important role in neuronal apoptosis. This gene is upregulated in neuronal degeneration and is observed in Alzheimer′s disease.

The primary function of prostate apoptosis response 4 protein (Par-4) is to mediate apoptosis. In neuronal progenitor cells, ceramide and PAR-4 contribute to rapid apoptosis. PAR-4 activates the tumor necrosis factor receptor superfamily member (FAS)-based death signalling caspase cascade to accomplish apoptosis in prostate tumor cells. PAR-4 is considered as a potential drug for treatment of cancer and neurodegenerative disorders.

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

For continuous use, store at 2-8 °C for up to one month.For prolonged storage, freeze in working aliquots at −20 °C. Repeated freezing and thawing is not recom-mended. Storage in frost-free freezers is also notrecommended. If slight turbidity occurs upon prolongedstorage, clarify the solution by centrifugation beforeuse. Working dilutions should be discarded if not usedwithin 12 hours.

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