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P5381

Phosphoglucose Isomerase from baker′s yeast (S. cerevisiae)

Name: Phosphoglucose Isomerase from baker′s yeast (<I>S. cerevisiae</I>)
Brand: SIGMA

Type III, ammonium sulfate suspension, ≥400 units/mg protein (biuret)

Synonym(s):

D-Glucose-6-phosphate ketol-isomerase, PGI, Phosphosaccharomutase

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About This Item

CAS Number:

9001-41-6

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-603-1

MDL number:

MFCD00131887

EC Number:

5.3.1.9 (BRENDA, IUBMB)

Specific activity:

≥400 units/mg protein (biuret)

Biological source:

bakers yeast

Concentration:

≥3 mg/mL protein Biuret

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Properties

biological source

bakers yeast

Quality Segment

200

type

Type III

form

ammonium sulfate suspension

specific activity

≥400 units/mg protein (biuret)

mol wt

tetramer 119,500 Da±600

concentration

≥3 mg/mL protein Biuret

technique(s)

activity assay: suitable

color

white

Protein ID accession no.

NP_009755.1

UniProt accession no.

P12709

storage temp.

2-8°C

Gene Information

yeast ... PGI1(852495)

Description

General description

Research area: Cell Signaling

Phosphoglucose Isomerase ubiquitous multifunctional cytosolic protein. This dimeric protein has a molecular weight of approximately 119,500 ± 600 and is composed of four subunits that are nearly identical.

Application

Phosphoglucose Isomerase from baker′s yeast (S. cerevisiae) has been used:

to convert fructose-6-phosphate to glucose-6-phosphate during the enzymatic measurement of serum mannose levels.
as one of the enzymes in the series of four enzyme-coupled reactions to measure phosphomannomutase (PMM) activity.
to convert fructose to glucose while evaluating fructose content in kudzu roots.

Biochem/physiol Actions

Phosphoglucose Isomerase(PGI) is an enzyme crucial for the interconversion of D-glucose 6-phosphate and D-fructose 6-phosphate. PGI is responsible for the second step of glycolysis and is involved in glucogenesis and pentose phosphate pathway (PPP). It is highly conserved in bacteria and eukaryotes. PGI plays a vital role in the survival of A. fumigatus and demonstrates diverse functions that encompass development, biosynthesis of cell wall glucan, and virulence.Phosphoglucose Isomerase functions as an isomerase, neuroleukin, autocrine motility factor, and a differentiation and maturation mediator.

Physical form

Crystalline suspension in 2.6 M (NH₄)₂SO₄ containing 0.1 mM magnesium acetate, pH 7.0

Other Notes

One unit will convert 1.0 μmole of D-fructose 6-phosphate to D-glucose 6-phosphate per min at pH 7.4 at 25 °C.

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Pictograms

GHS08

Signal Word

Danger

Hazard Codes

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

10 - Combustible liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)