P6611
HIS-Select® Nickel Affinity Gel
(1:1 suspension in a 20% ethanol solution)
Synonym(s):
Ni-NTA resin, nickel charged agarose
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About This Item
NACRES:
NA.56
UNSPSC Code:
12352200
conjugate
magnetic beads
Quality Segment
form
(1:1 suspension in a 20% ethanol solution)
feature
hydrophilic
packaging
pkg of 1 mL, pkg of 100 mL, pkg of 25 mL, pkg of 5 mL, pkg of 500 mL
concentration
1.5-2.4 mL/mL (suspension in packed gel)
technique(s)
protein purification: suitable
color
faint blue to very dark blue
matrix
6% Beaded Agarose
capacity
>15 mg/mL, gel binding capacity (protein)(with an approx. 30 kDa protein)
transition temp
flash point 32 °C (closed cup)
storage temp.
2-8°C
General description
HIS-Select® Nickel Affinity Gel is an immobilized metal ion affinity chromatography (IMAC) product, used for the purification of His-tagged proteins. While the unique, non-charged, hydrophilic linkage of the proprietary quadridentate NTA chelate group to the beaded agarose charged with nickel ensures high selectivity for small to medium scale His-tag protein purification, it also results in reduced non-specific binding of other proteins. HIS-Select Nickel Affinity Gel is selective for recombinant proteins with His-tags and exhibits low non-specific binding of other proteins. The selectivity can be modulated with the inclusion of imidazole during chromatography.
Application
HIS-Select® Nickel Affinity Gel has been used in the purification of recombinant proteins like EF-hand calcium-binding protein (S100A14), LIM homeobox transcription factor 1 alpha protein, sigma-1 receptor as well as harpin, stable protein 1 (SP1), and BCR-ABL fusion protein.
Features and Benefits
- High selectivity for higher purity.
- Unique non-charged hydrophilic linkage reduces non-specific binding.
- Binding capacity for histidine-tagged protein is greater than 15 mg/mL.
- Binding under denaturing or non-denaturing conditions.
- One-step purification.
- Minimizes unwanted ionic interactions.
- Minimal nickel leaching.
- Bead size: 45-165 μm.
Physical form
1:1 suspension in a 20% ethanol solution
Preparation Note
HIS-Select Nickel Affinity Gel is stable for at least one year when stored properly. The HIS-Select Nickel Affinity Gel should be cleaned after each use and an antimicrobial agent such as 20% ethanol should be added to the storage buffer.
Other Notes
It is also available with the EZview™ technology (Product Code E3528).
Legal Information
HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany
signalword
Warning
hcodes
Hazard Classifications
Flam. Liq. 3
Storage Class
3 - Flammable liquids
wgk
WGK 3
flash_point_f
89.6 °F - closed cup
flash_point_c
32 °C - closed cup
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