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P6635

Phosphorylase b from rabbit muscle

Name: Phosphorylase b from rabbit muscle
Brand: SIGMA

lyophilized powder, ≥20 units/mg protein, 2× crystallization

Synonym(s):

α-Glucan Phosphorylase, 1,4-α-D-Glucan:orthophosphate α-D-glucosyltransferase, Glycogen Phosphorylase

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About This Item

CAS Number:

9012-69-5

UNSPSC Code:

12352204

NACRES:

NA.77

EC Number:

232-737-0

MDL number:

MFCD00131905

EC Number:

2.4.1.1 (BRENDA, IUBMB)

Specific activity:

≥20 units/mg protein

Biological source:

rabbit muscle

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Properties

biological source

rabbit muscle

Quality Segment

300

form

lyophilized powder

specific activity

≥20 units/mg protein

mol wt

97,200 Da by calculation

purified by

2× crystallization

storage condition

(Keep container tightly closed in a dry and well-ventilated place)

technique(s)

mass spectrometry (MS): suitable

impurities

~0.01 μmol/mg protein 5′-AMP (This low level will not interfere with phosphorylase and phosphorylase kinase assays.)

UniProt accession no.

A0A5F9CLZ9, A0A5F9CRR0, A0A5F9D1C4, A0A5F9DLL9

foreign activity

phosphoglucomutase ≤1.0%, phosphorylase a ≤10%, phosphorylase kinase ≤0.5%, phosphorylase phosphatase, debrancher enzyme, AMPase and ATPase ≤0.1%

storage temp.

−20°C

Description

General description

Research area: Cell Signaling

Glycogen phosphorylase (PG), a specialized complex allosteric enzyme has an evolutionarily conserved gene sequence. GP contains a family of three isozymes such as muscle GP (mGP), liver GP (lGP), and brain GP (bGP) in humans.

Application

Phosphorylase b from rabbit muscle has been used:

in the calibration of Sepharose C1-6B columns while studying the molecular weight of methylamine dehydrogenase subunits
in ion mobility-mass spectrometry studies of phosphorylase B ions that have been generated with supercharging reagents, in the charge-reducing buffer
for the preparation of p³² labeled phosphorylase A using phosphorylase kinase and [³²P]ATP
in phosphorylase phosphatase assay
in enzyme assay as a positive control to ensure the reaction system for the activity determination was adopted

Biochem/physiol Actions

Phosphorylase b is a non-active form and is present in resting muscles. Phosphorylase b kinase activity increases significantly when the Mg²⁺:ATP ratio exceeds 1. The breakdown of ATP during muscle contraction is thought to trigger in vivo conversion of phosphorylase b into a. Phosphorylase b is activated by inosine monophosphate. Glycogen phosphorylase (PG) enzyme plays a vital role in the first step of glycogenolysis. In the initial stage of glycogenolysis, glycogen phosphorylase(GP) breaks α-1,4- -glycosidic bonds, releasing the glucose-1-phosphate (G1P)molecule. When incubated with the proper concentrations of glucose-1-phosphate without the addition of primer, rabbit muscle phosphorylase B was found to be capable of forming protein-bound alpha-1,4 glucosyl chains.

Packaging

Package size based on protein content.

Physical form

Lyophilized powder containing lactose, 5′-AMP, and Mg(OAc)₂ (10 μmole per 100 mg protein)

Other Notes

One unit will form 1.0 μmole of α-D-glucose 1-phosphate from glycogen and orthophosphate in the presence of 5′-AMP, per min at pH 6.8 at 30 °C measured in a system containing phosphoglucomutase, NADP, and glucose 6-phosphate dehydrogenase. (One μmolar unit is equivalent to approx. 45 Cori units.)

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c