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Merck
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P6847

Monoclonal Anti-Interleukin-2−R-Phycoerythrin antibody produced in mouse

clone 5334, purified immunoglobulin, buffered aqueous solution

Synonym(s):

Monoclonal Anti-Interleukin-2, Monoclonal Anti-IL-2

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About This Item

NACRES:
NA.41
UNSPSC Code:
51111800
Conjugate:
phycoerythrin (R-PE) conjugate
Clone:
5334, monoclonal
Application:
FACS
Citations:
3

Key Documents

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biological source

mouse

conjugate

phycoerythrin (R-PE) conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

5334, monoclonal

form

buffered aqueous solution

species reactivity

human

technique(s)

flow cytometry: suitable

isotype

IgG1

UniProt accession no.

P60568

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... IL2(3558)

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General description

Interleukin-2 (IL-2) also refers as T Cell Growth Factor is a cytokine produced by certain subsets of T lymphocytes. It plays various functions like inducing interferon γ and B cell growth factor secretion as well as affecting the activation and proliferation of NK cells. It also has a vital role in modulating the expression of the IL-2 receptor and can facilitate B cell growth and differentiation. Mouse monoclonal anti-Interleukin-2−R-Phycoerythrin antibody reacts specifically with human IL-2.

Immunogen

recombinant human IL-2

Application

Monoclonal anti-Interleukin-2−R-Phycoerythrin antibody can be used in flow cytometry assays for identifying intracytoplasmic cytokines and for enumerating cytokine producing cells present in a mixed population.

Biochem/physiol Actions

The conjugates will identify and quantitate cells containing intracellular forms of human IL-2 by flow cytometry.

Physical form

Solution in buffered saline containing 0.5% bovine serum albumin and 0.1% sodium azide.

Preparation Note

Purified antibody is conjugated to R-phycoerythrin (PE).

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
SLBB8259V
119H0123
053K1269
044K1489

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Selective in vivo growth of T lymphocytes from normal human bone marrows.
Morgan DA
Science, 193(4257), 1007-1008 (1967)
T A Waldmann et al.
The Journal of experimental medicine, 160(5), 1450-1466 (1984-11-01)
Using anti-Tac, a monoclonal anti-interleukin 2 (IL-2) receptor antibody, we have explored the possibility that certain activated B cells display receptors for IL-2. Resting normal B cells and unselected B cell lines established from normal individuals were Tac antigen negative.
K A Smith et al.
Proceedings of the National Academy of Sciences of the United States of America, 82(3), 864-868 (1985-02-01)
The cell surface density of high-affinity membrane receptors for the T-lymphocytotrophic hormone interleukin 2 (IL-2) determines the rate of T-cell-cycle progression. Since 10-fold greater numbers of IL-2 receptor molecules were found by using a radiolabeled monoclonal antibody reactive with IL-2

Related Content

Data Sheet - P6847

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