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P7000

Sigma-Aldrich

Pepsin from porcine gastric mucosa

powder, ≥250 units/mg solid

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Synonym(s):
Pepsin A, Pepsin from hog stomach
CAS Number:
9001-75-6
Enzyme Commission number:
3.4.23.1 (BRENDA, IUBMB)
EC Number:
232-629-3
MDL number:
MFCD00081840
eCl@ss:
42010127

biological source

Porcine gastric mucosa

Quality Level

200

form

powder

specific activity

≥250 units/mg solid

mol wt

35 kDa

UniProt accession no.

P00791

application(s)

diagnostic assay manufacturing

shipped in

wet ice

storage temp.

2-8°C

Gene Information

pig ... LOC396892(396892)

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Application

Pepsin is a peptidase used to digest proteins and is commonly used in the preparation of Fab fragments from antibodies. Pepsin, from porcine gastric mucosa, has been used to hydrolyze dry cervical samples in mice. Product P7000 is provided as a powder and has been used to treat epithelial cells from a single feline mammary carcinoma.
The enzyme from Sigma has been used to simulate in vitro gastric digestion of cooked cod. It has been used to simulate in vitro gastric digestion of cocoa mass and supplemented dietary fiber. It has also been used to increase the fraction of extractable soluble collagen and to lower the immunogenicity of the resulting collagen from bovine dermal tissue.
Pepsin cleavage can be used to produce F(ab′)2 fragments of antibodies. pepsin at www.sigma-aldrich.com/enzymeexplorer.

Biochem/physiol Actions

Pepsin hydrolyzes peptide bonds, not amide or ester linkages. Pepsin cleaves peptides with an aromatic acid on either side of the peptide bond. Sulfur-containing amino acids increase susceptibility to hydrolysis when they are close to the peptide bond. Pepsin preferentially cleaves at the carboxyl side of phenylalanine and leucine and at the carboxyl side of glutamic acid residues. Cleaves Phe-Val, Gln-His, Glu-Ala, Ala-Leu, Leu-Tyr, Tyr-Leu, Gly-Phe, Phe-Phe and Phe-Tyr bonds in the β chain of insulin
Pepsin is the major proteolytic enzyme produced in the stomach. It digests proteins through the cleavage of interior peptide linkages..
The enzyme does not cleave at valine, alanine, or glycine linkages. Z-L-tyrosyl-L-phenylalanine, Z-L-glutamyl-L-tyrosine, and Z-L-methionyl-L-tyrosine may be used as substrates for pepsin digestion. Pepsin is inhibited by several phenylalanine-containing peptides.
Preferential cleavage: hydrophobic and aromatic residues in P1 and P1′ postitions. Cleaves Phe-Val, Gln-His, Glu-Ala, Ala-Leu, Leu-Tyr, Tyr-Leu, Gly-Phe, Phe-Phe and Phe-Tyr bonds in the β chain of insulin

Unit Definition

One unit will produce a ΔA280 of 0.001 per min at pH2.0 at 37 °C, measured as TCA-soluble products using hemoglobin as substrate. (Final volume = 16ml. Light path = 1cm.)

Analysis Note

Optimum pH is 2-4. Active in 4 M urea and 3 M guanidine HCl. Stable at 60 °C. Pepsin is irreversibly inactivated at pH 8.0 - 8.5.

Other Notes

View more information on pepsin at www.sigma-aldrich.com/enzymeexplorer.

inhibitor

Product No.
Description
Pricing

Pictograms

Exclamation markHealth hazard
GHS07,GHS08

Signal Word

Danger

Hazard Statements

H315 - H319 - H334 - H335

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. I've seen the term "1:10000" used to define Product P7000, Pepsin from porcine gastric mucosa  (Pepsin A). What does that mean?

    A historical measurement of pepsin activity was the ratio of pepsin to coagulated egg white that the pepsin would digest. Thus 1:10,000 would refer to one part pepsin to 10,000 parts coagulated egg white.  More recent analyses use hemoglobin as a substrate, in place of coagulated egg white.

  4. Can I freeze stock solutions of Product P7000, Pepsin from porcine gastric mucosa  (Pepsin A)?

    Solutions of the enzyme at pH 4.4 should be stable at -20 °C for 2-3 months.

  5. Product P7000, Pepsin from porcine gastric mucosa  (Pepsin A), can be stored at room temperature, but other pepsins are stored in the cooler or freezer.  Why is this?

    This relates to autodigestion of pepsin.  P7000 is a relatively "crude" pepsin, where other proteins that are present would be more likely digestion targets.  With more purified pepsin, the risk of autodigestion increases, and storage at colder temperatures reduces that risk.

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  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

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Investigation of selenium speciation in in vitro gastrointestinal extracts of cooked cod by high-performance liquid chromatography-inductively coupled plasma mass spectrometry and electrospray mass spectrometry.
JohnaLewis, D.
Journal of Analytical Atomic Spectrometry, 11(12), 1177-1182 (1177)
Harri Mäkivuokko et al.
Bioscience, biotechnology, and biochemistry, 71(8), 1834-1843 (2007-08-11)
Effects of cocoa mass and supplemented dietary fiber (polydextrose) on microbial fermentation were studied by combining digestion simulations of stomach and small intestine with multi-staged colon simulations. During the four phases of digestion, concentrations of available soluble proteins and reducing
Robert J Kane et al.
Journal of the mechanical behavior of biomedical materials, 7, 41-49 (2012-02-22)
Freeze-dried collagen scaffolds reinforced with hydroxyapatite (HA) are of clinical interest for synthetic bone graft substitutes and tissue engineering scaffolds, but a systematic evaluation of the effects of the HA reinforcement weight fraction and morphology on the mechanical properties is
Dixon, M., et al.
Enzymes, 262-262 (1979)
Enzymes of Molecular Biology
Sweeney, P.J., and Walker, J.M.
Methods in Molecular Biology, 290-291 (1993)
View All Related Papers

Protocols

Enzymatic Assay of Pepsin (3.4.23.1)

This procedure may be used for determination of Pepsin activity using hemoglobin as the substrate. It is a spectrophotometric stop rate determination.

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