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P7119

Sigma-Aldrich

PGO Enzyme Preparation

1 G capsules

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Synonym(s):
Peroxidase - Glucose oxidase preparation
NACRES:
NA.54

Quality Level

200

form

powder

solubility

H2O: soluble capsule/100 mL at 25 °C, clear, colorless (pH 6.95-7.05)

storage temp.

2-8°C

General description

Each capsule contains 500 units of glucose oxidase (Aspergilus niger), 100 purpurogallin units of peroxidase (horseradish), and buffer salts. The reaction produces oxidized o-Dianisidine, which is brown. The intensity of the brown color measured at 425-475 nm is proportional to the original glucose concentration.

Application

PGO Enzymes preparation has been used:
  • in diet sampling and chemical analyses
  • to determine plasma and follicular fluid glucose levels
  • to measure glucose content in fractions of retrograded debranched rice starch

Biochem/physiol Actions

PGO Enzymes are used for the quantitative, enzymatic determination of glucose in aqueous solutions such as serum. The reactions are normally monitored at 425-475 nm utilizing o-dianisidine as a colorimetric substrate. Glucose oxidase methods eliminate the effects of interfering substances. Contents of one capsule includes 100 units peroxidase and 500 units glucose oxidase and buffer salts.

Suitability

Tested and found suitable for use in the quantitation of glucose.

Preparation Note

The PGO enzymes solution is prepared by adding the contents of 1 capsule of PGO enzymes to 100 mL of water in an amber bottle. Bottle should be inverted several times with gentle shaking to dissolve. PGO Enzymes solution should be stored at 2-8 °C. The solution is stable for up to 1 month unless turbidity develops, and for at least 6 months at –20 °C.

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

含少量动物源组分生物产品
常规特殊物品

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