P7173
Pyruvate Carboxylase from bovine liver
buffered aqueous glycerol solution, 5-25 units/mg protein (BCA)
Synonym(s):
Pyruvate:CO2 ligase (ADP-forming)
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.26
MDL number:
Specific activity:
5-25 units/mg protein (BCA)
Concentration:
≥0.5 mg/mL
Quality Segment
form
buffered aqueous glycerol solution
specific activity
5-25 units/mg protein (BCA)
concentration
≥0.5 mg/mL
foreign activity
lactic dehydrogenase ≤0.5%
storage temp.
−20°C
Application
The enzyme from Sigma has been used as a positive control during the assay of pyruvate carboxylase activity in cell-free extracts of Corynebacterium glutamicum.
Pyruvate is critical for gluconeogenesis, lipogenesis, glyceroneogenesis, neurotransmitter biosynthesis and glucose-induced insulin, and is used to study these processes.
Biochem/physiol Actions
Pyruvate carboxylase catalyzes the carboxylation of pyruvate to oxaloacetate. Pyruvate carboxylase is a mitochondrial protein that has a biotin prosthetic group that requiries magnesium or manganese and acetyl CoA.
Physical form
Solution in 50% glycerol containing 0.05 M Tris-HCl, pH 7.4, 2 mM magnesium acetate and 1 mM EDTA.
Preparation Note
Affinity purified
Other Notes
One unit will convert 1.0 μmole of pyruvate and CO2 to oxalacetate per min at pH 7.8 at 30 °C.
Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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