biological source
synthetic
Assay
≥98.5% (GC)
form
powder
mp
91-94 °C
solubility
dry solvents (ethanol, methanol, and 2-propanol): 200 mM (Stock solution are stable for months at 4°C.)
H2O: unstable
SMILES string
FS(=O)(=O)Cc1ccccc1
InChI
1S/C7H7FO2S/c8-11(9,10)6-7-4-2-1-3-5-7/h1-5H,6H2
InChI key
YBYRMVIVWMBXKQ-UHFFFAOYSA-N
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General description
Application
Noted general features and benefits of PMSF include the following:
- Inhibits serine proteases such as trypsin and chymotrypsin
- Also inhibits cysteine proteases (reversible by reduced thiols) and mammalian acetylcholinesterase
- Not as effective or as toxic as DFP
- Effective concentration 0.1-1 mM
- Half-life = 1 hr. at pH 7.5
- cell fractionation.
- used as a supplement in nuclear protein extraction.
- inhibitor of cholesterol esterase (CE) and pseudocholinesterase (PCE).
- used for the collection of blood prior to centrifugation to quantify plasma ANP levels.
Biochem/physiol Actions
related product
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 3 Oral - Skin Corr. 1B
Storage Class Code
6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
Certificates of Analysis (COA)
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705578-5MG-PW
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1000309185
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Aldrich Products
For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').
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How should Product P7626, Phenylmethanesulfonyl fluoride (PMSF), solutions be prepared?
Stock solutions of 100-200 mM can be prepared in anhydrous isopropanol or anhydrous absolute ethanol. PMSF is unstable in the presence of water.
How should stock solutions of Product P7626, Phenylmethanesulfonyl fluoride (PMSF), be stored?
A 200 mM solution in dry solvent is stable for at least 9 months when stored at 2-8°C.
What does Product P7626, Phenylmethanesulfonyl fluoride (PMSF), inhibit?
PMSF is a serine protease inhibitor, inhibiting the activity of enzymes such as thrombin, trypsin, papain, chymotrypsin and proteinase K. It also inhibits mammalian acetylcholinesterases.
What is the effective amount of Product P7626, Phenylmethanesulfonyl fluoride (PMSF), to be used?
The effective concentration range is 0.1-1 mM. A final concentration of 5 mM was used for proteinase K inhibition.
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
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