P7899
Anti-Peroxidase antibody produced in rabbit
fractionated antiserum, lyophilized powder
Synonym(s):
Rabbit Anti HRP
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About This Item
Conjugate:
unconjugated
Clone:
polyclonal
Application:
indirect ELISA
Species reactivity:
horseradish
Citations:
36
Technique(s):
indirect ELISA: 1:100,000-1:200,000
Product Name
Anti-Peroxidase antibody produced in rabbit, fractionated antiserum, lyophilized powder
biological source
rabbit
conjugate
unconjugated
antibody form
fractionated antiserum
antibody product type
primary antibodies
clone
polyclonal
form
lyophilized powder
species reactivity
horseradish
technique(s)
indirect ELISA: 1:100,000-1:200,000
storage temp.
2-8°C
target post-translational modification
unmodified
Quality Level
Analysis Note
Specificity is determined by immunoelectrophoresis using a crude peroxidase extract.
Application
Anti-Peroxidase antibody produced in rabbit has been used in:
- immunoblot assays
- enzyme-linked-immunosorbent assay (ELISA)
- immunoprecipitation
Biochem/physiol Actions
Peroxidase helps in catalyzing the oxidation of a number of substrates with the help of hydrogen peroxide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Antiserum is determined to be immunospecific for horseradish peroxidase and does not inhibit the enzymatic activity of the peroxidase.
Peroxidase is a hemoprotein.
The fractionation procedure yields primarily the immunoglobulin fraction of antiserum. If necessary, the fractionated antiserum is adsorbed using solid phase techniques. Antiserum is determined to be immunospecific by immunoelectrophoresis (IEP), against purified and crude horseradish peroxidase
The gel is stained with DAB (diaminobenzidine) to ensure that the antibody does not inhibit the enzymatic activity of the peroxidase. Identity and purity of the antibody is established by immunoelectrophoresis. Electrophoresis of the antibody preparation followed by diffusion against anti-rabbit IgG and anti-rabbit whole serum results in single arcs of precipitation in the γ region
Each mL will precipitate a minimum of 0.25 mg of peroxidase at equivalence. May be used to stain axons and synaptic terminals in Drosophila embryo fillets.
The gel is stained with DAB (diaminobenzidine) to ensure that the antibody does not inhibit the enzymatic activity of the peroxidase. Identity and purity of the antibody is established by immunoelectrophoresis. Electrophoresis of the antibody preparation followed by diffusion against anti-rabbit IgG and anti-rabbit whole serum results in single arcs of precipitation in the γ region
Each mL will precipitate a minimum of 0.25 mg of peroxidase at equivalence. May be used to stain axons and synaptic terminals in Drosophila embryo fillets.
Immunogen
Purified peroxidase from horseradish
Physical form
Lyophilized from 0.01 M phosphate buffered saline, pH 7.2
Preparation Note
Reconstitute with deionized water.
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
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Oxidative stress
Biochemical Ecotoxicology. Principles and Methods (2014)
Michael P Hollandsworth et al.
Brain research, 1298, 131-144 (2009-09-08)
The anterior ethmoidal nerve (AEN) innervates the nasal passages and external nares, and serves as the afferent limb of the nasopharyngeal and diving responses. However, although 65% of the AEN is composed of unmyelinated fibers, it has not been determined
Peroxidase activity of biopolymer complex isolated from Aloe barbadensis Miller
Vittori N
International journal of biochemistry and biotechnology, 3(2-3), 105-117 (2007)
Prachi Jain et al.
PloS one, 13(5), e0197132-e0197132 (2018-05-17)
Present work focuses on tissue and concentration-dependent effect of nitric oxide (NO) on the modulation of cytosolic peroxidase (POD; EC 1.11.1.7) activity in 2-day old etiolated sunflower (Helianthus annuus L.) seedlings. Exogenously supplied NO (in the form of sodium nitroprusside
Megan L Mickum et al.
Infection and immunity, 84(5), 1371-1386 (2016-02-18)
Infection of mammals by the parasitic helminth Schistosoma mansoni induces antibodies to glycan antigens in worms and eggs, but the differential nature of the immune response among infected mammals is poorly understood. To better define these responses, we used a
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