P7937
Protein Phosphatase-1 Catalytic Subunit, α-Isoform from rabbit
5,000-15,000 units/mg protein, recombinant, expressed in E. coli, lyophilized powder
Synonym(s):
Protein phosphatase
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About This Item
Specific activity:
5,000-15,000 units/mg protein
Assay:
≥80% (GE)
Biological source:
rabbit
Recombinant:
expressed in E. coli
biological source
rabbit
Quality Segment
recombinant
expressed in E. coli
assay
≥80% (GE)
form
lyophilized powder
specific activity
5,000-15,000 units/mg protein
mol wt
37.5 kDa
technique(s)
inhibition assay: suitable
UniProt accession no.
storage temp.
−20°C
Gene Information
rabbit ... PPP1CA(100009298)
General description
Protein phosphatase1 (PP1) is a heterodimeric enzyme with serine/threonine phosphatase activity. It comprises a catalytic subunit and a targeting subunit or a specific protein inhibitor. The targeting subunit determines the enzyme localization, e.g., to glycogen particles (subunit G) or to myofibrils (subunit M).
Application
Protein Phosphatase-1 Catalytic Subunit, α-Isoform from rabbit has been used as a standard in protein phosphatase-1 inhibition assay.
Protein Phosphatase-1 Catalytic Subunit, α-Isoform from rabbit has been used in protein phosphatase reaction and in the digestion of adipocyte extracts.
Biochem/physiol Actions
Protein phosphatase1 (PP1) is involved in glycogen metabolism and in regulation of muscle contractility. In addition, it is implicated in cell cycle and transcriptional regulation. Recombinant rabbit PP1α has properties in common with the native rabbit muscle protein including size, requirement for Mn2+ for activity, specificity for phosphorylase A, and inhibition by okadaic acid, microcystin LR, and phosphatase inhibitor2 (I-2).
Packaging
Package size based on protein content.
Physical form
Lyophilized powder containing imidazole buffer, pH 7.4, NaCl, DTT, EDTA, MnCl2, Tween 20, and trehalose as stabilizer.
Other Notes
One unit will hydrolyze 1 nmole of p-nitrophenyl phosphate per min at pH 7.4 at 30 °C.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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