Merck
CN
All Photos(2)

Documents

Safety Information

P8215

Sigma-Aldrich

Protease Inhibitor Cocktail

for use with fungal and yeast extracts, DMSO solution

Sign Into View Organizational & Contract Pricing
All Photos(2)
Synonym(s):
Protease Inhibitor Mix, Protease inhibitor
EC Number:
200-664-3
MDL number:
MFCD00677817

Quality Level

300

form

DMSO solution

storage temp.

−20°C

Looking for similar products? Visit Product Comparison Guide

Related Categories

General description

The Protease Inhibitor Cocktail for fungal and yeast extracts is a mixture of protease inhibitors in DMSO solution. The cocktail has been optimized and tested for use on fungal and yeast samples, specifically on Saccharomyces cerevisiae cells. One mL of the cocktail is recommended for the inhibition of proteases extracted from 20 g of yeast.

Specificity

Inhibits serine, cysteine, aspartic, and metalloproteases.

Application

Protease Inhibitor Cocktail has been used as a component in:
  • FA buffer to wash spheroplasts to obtain an average DNA fragment size for chromatin immunoprecipitation (chip)
  • potassium phosphate buffer (pbs) to digest chitin
  • extraction buffer for grinding powdered mycelia for co-immunoprecipitation analysis

Features and Benefits

  • Broad specificity: inhibits a wide range of proteases, providing comprehensive protection to fungal and yeast extracts.
  • Tested on Saccharomyces cerevisiae cells: the cocktail has been optimized for use on this commonly studied yeast strain.
  • Convenient packaging: available in a 1 or 5 mL glass bottle for easy handling and storage.
  • Ready-to-use solution: the cocktail is supplied in DMSO solution for immediate use in protease inhibition assays.
  • Effective inhibition: each component in the cocktail has been carefully selected for its specific inhibitory properties, ensuring reliable and consistent results.

Components

AEBSF, 100 mM
E-64, 1.4 mM
Pepstatin A, 2.2 mM
1,10-Phenanthroline, 500 mM

Other Notes

Mixture of protease inhibitors with broad specificity for the inhibition of serine, cysteine, aspartic and metallo-proteases. Contains 4-(2-aminoethyl)benzenesulfonyl fluoride (AEBSF), pepstatin A, E-64, and 1,10-phenanthroline.
This product is for R&D use only, not for drug, household, or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.

Quantity

One mL is recommended for the inhibition of proteases extracted from 20 g of yeast.

Physical form

Solution in DMSO

related product

Product No.
Description
Pricing

P8465

Protease Inhibitor Cocktail powder, for use with bacterial cell extracts, lyophilized powder

P8340

Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution

P2714

Protease Inhibitor Cocktail powder, for general use, lyophilized powder

P8849

Protease Inhibitor Cocktail, for use in purification of Histidine-tagged proteins, DMSO solution

P9599

Protease Inhibitor Cocktail, for plant cell and tissue extracts, DMSO solution

P1860

Protease Inhibitor Cocktail, for use in tissue culture media, DMSO solution

S8820

SIGMAFAST™ Protease Inhibitor Tablets, For General Use

S8830

SIGMAFAST™ Protease Inhibitor Cocktail Tablets, EDTA-Free, for use in purification of Histidine-tagged proteins

Pictograms

Exclamation markEnvironment
GHS07,GHS09

Signal Word

Warning

Hazard Statements

H302 - H315 - H319 - H410

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Irrit. 2 - Skin Irrit. 2

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

188.6 °F

Flash Point(C)

87 °C

Regulatory Information

常规特殊物品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

A genome-wide screen reveals a role for the HIR histone chaperone complex in preventing mislocalization of budding yeast CENP-A
Ciftci-Yilmaz S, et al.
Genetics, 210(1), 203-218 (2018)
Takashi Kubota et al.
Molecular & cellular proteomics : MCP, 10(7), M110-M110 (2011-04-21)
Yeast cells lacking Ctf18, the major subunit of an alternative Replication Factor C complex, have multiple problems with genome stability. To understand the in vivo function of the Ctf18 complex, we analyzed chromatin composition in a ctf18Δ mutant using the
Elena Pérez-Nadales et al.
The Plant cell, 23(3), 1171-1185 (2011-03-29)
Fungal pathogenicity in plants requires a conserved mitogen-activated protein kinase (MAPK) cascade homologous to the yeast filamentous growth pathway. How this signaling cascade is activated during infection remains poorly understood. In the soil-borne vascular wilt fungus Fusarium oxysporum, the orthologous
Jin H Lee et al.
Virology, 378(2), 347-354 (2008-07-08)
In this study we have defined protein-protein interactions between the structural proteins of herpes simplex virus type 1 (HSV-1) using a LexA yeast two-hybrid system. The majority of the capsid, tegument and envelope proteins of HSV-1 were screened in a
Wei Xie et al.
Molecular biology of the cell, 20(14), 3317-3329 (2009-05-22)
Endoplasmic reticulum (ER) quality control mechanisms monitor the folding of nascent polypeptides of the secretory pathway. These are dynamic processes that retain folding proteins, promote the transport of conformationally mature proteins, and target misfolded proteins to ER-associated degradation (ERAD) pathways.
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service