P8811
Protease from Streptomyces griseus
powder, BioReagent, suitable for mouse embryo cell culture, ≥3.5 units/mg solid
Synonym(s):
Actinase E, Pronase E
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About This Item
CAS Number:
UNSPSC Code:
12352207
EC Number:
232-909-5
NACRES:
NA.75
eCl@ss:
32160410
MDL number:
Form:
powder
product line
BioReagent
Quality Level
form
powder
specific activity
≥3.5 units/mg solid
technique(s)
cell culture | embryo: suitable
impurities
starch, essentially free
storage temp.
−20°C
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General description
Completely inactivated by heating above 80 °C for 15-20 minutes.
Application
Protease is typically used in nucleic acid isolation procedures in incubations of 0.5-3.0 hours supplemented with 0.2% sodium dodecyl sulfate and 10 mM EDTA.
This enzyme is more active at a higher pH range than the known alkaline protease, showing the proteolytic activity even in 0.2N NaOH solution. This enzyme is useful for proteolysis of insoluble protein and for structure investigation of protein.
Biochem/physiol Actions
A mixture of at least three proteolytic activities including an extracellular serine protease. In general, serine proteases display a wide range of substrate specificities, which are believed to be mediated by an active site composed of one Asp, one His, and a Ser residue in the molecule. This enzyme prefers to hydrolyze peptide bonds on the carboxyl side of glutamic or aspartic acid.
Preparation Note
Collected from culture broth of S. griseus.
Analysis Note
Contains approximately 25% calcium acetate.
Other Notes
An unusually non-specific protease.
One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
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S Blumberg et al.
European journal of biochemistry, 136(1), 151-154 (1983-10-17)
A series of 2-mercaptoacetyl-dipeptides, a potential group of metalloendopeptidase inhibitors, has been synthesized by coupling the N-hydroxysuccinimide ester of S-acetyl-2-mercaptoacetic acid with hydrophobic dipeptide methyl ester hydrochlorides, followed by hydrolysis with NaOH in aqueous methanol and acidification with HCl. Thus
Shantha K Mahadevaiah et al.
Nature, 586(7830), 612-617 (2020-08-21)
Single-cell RNA sequencing of embryos can resolve the transcriptional landscape of development at unprecedented resolution. To date, single-cell RNA-sequencing studies of mammalian embryos have focused exclusively on eutherian species. Analysis of mammalian outgroups has the potential to identify deeply conserved
J Erik Mylroie et al.
Environmental toxicology and chemistry, 40(3), 780-791 (2020-10-13)
Perfluorooctanesulfonic acid (PFOS) is a perfluorinated compound used in many industrial and consumer products. It has been linked to a broad range of adverse effects in several species, including zebrafish (Danio rerio). The zebrafish embryo is a widely used vertebrate
Yan Jiang et al.
Human gene therapy, 30(2), 179-196 (2018-07-20)
Hepatic stellate cells (HSCs) are the primary cell type responsible for liver fibrogenesis. Transforming growth factor beta 1 (TGF-β1) and platelet-derived growth factor (PDGF) are key profibrotic cytokines that regulate HSC activation and proliferation with functional convergence. Dual RNA interference
Archana Vijaya Kumar et al.
Cancer science, 111(8), 2907-2922 (2020-06-24)
Heparan sulfate proteoglycans (HSPGs) act as signaling co-receptors by interaction of their sulfated glycosaminoglycan chains with numerous signaling molecules. In breast cancer, the function of heparan sulfate 2-O-sulfotransferase (HS2ST1), the enzyme mediating 2-O-sulfation of HS, is largely unknown. Hence, a
Articles
Mouse embryo media and embryo validated reagents for transgenic mouse embryo culture
用于转基因小鼠胚胎培养的小鼠胚胎培养基及经过胚胎培养验证的试剂
Global Trade Item Number
| SKU | GTIN |
|---|---|
| P8811-1G | 04061834401834 |
| P8811-100MG | 04061835569311 |
| P8811-5G | 04061835507368 |
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