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P8913

Sigma-Aldrich

Phospholipase A2 from bovine pancreas

lyophilized powder, ≥20 units/mg protein

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Synonym(s):
Lecithinase A, PLA2, Phosphatidylcholine 2-acylhydrolase
CAS Number:
9001-84-7
Enzyme Commission number:
3.1.1.4 (BRENDA, IUBMB)
EC Number:
232-637-7
MDL number:
MFCD00131890
NACRES:
NA.32

form

lyophilized powder

Quality Level

200

specific activity

≥20 units/mg protein

composition

Protein, 5-20% biuret

UniProt accession no.

P00593

storage temp.

−20°C

Gene Information

cow ... PLA2G1B(282457)

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General description

Phospholipase A2 from bovine pancreas is a calcium-dependent lipolytic enzyme.

Application

Phospholipase A2 from bovine pancreas has been used to determine rat renal proximal tubular segments (PTS) viability during oxygenation and hypoxia-reoxygenation and to study the impact of phospholipase A2 on tubular cell triglyceride levels.

Biochem/physiol Actions

Hydrolyzes the β-ester bond of zwitterionic glycerophospholipids. Preferred substrates are phosphatidylcholine, phosphatidylethanolamine, and their plasmalogen analogues. Phosphatidylinositol and phosphatidylserine are also hydrolyzed. Aggressively attacks phospholipids in membranes of intact cells.

Packaging

Package size based on protein content

Unit Definition

One unit will hydrolyze 1.0 μmole of soybean L-α-phosphatidylcholine to L-α-lysophosphatidylcholine and a fatty acid per min at pH 8.0 and 37 °C.

Physical form

Contains Tris buffer salts.

Analysis Note

Protein determined by biuret.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Triglyceride accumulation in injured renal tubular cells: alterations in both synthetic and catabolic pathways.
Johnson AC
Kidney International, 67(6), 2196-2209 (2005)
Renal tubular triglyercide accumulation following endotoxic, toxic, and ischemic injury.
Zager RA
Kidney International, 67(1), 111-121 (2005)
Phospholipase A2 activity can protect renal tubules from oxygen deprivation injury.
Zager RA
Proceedings of the National Academy of Sciences of the USA, 90(17), 8297-8301 (1993)
Ming Chen et al.
Scientific reports, 7(1), 15931-15931 (2017-11-23)
Hepatitis C virus (HCV), dengue virus (DENV) and Japanese encephalitis virus (JEV) belong to the family Flaviviridae. Their viral particles have the envelope composed of viral proteins and a lipid bilayer acquired from budding through the endoplasmic reticulum (ER). The
Intracellular phospholipases A.
H van den Bosch
Biochimica et biophysica acta, 604(2), 191-246 (1980-09-30)
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