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P8920

Poly-L-lysine solution

0.1 % (w/v) in H2O

Synonym(s):

2-(4-Cyanoanilino)-4,6-dichloropyrimidine, 4-[(4,6-Dichloro-2-pyrimidinyl)amino]benzonitrile

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About This Item

CAS Number:
25988-63-0
UNSPSC Code:
41116121
eCl@ss:
32160406
NACRES:
NA.47
MDL number:
MFCD00165424
Biological source:
synthetic
Mol wt:
150,000-300,000
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biological source

synthetic

Quality Level

200

shelf life

Expiry date on the label.

mol wt

150,000-300,000

concentration

0.1 % (w/v) in H2O

color

colorless

application(s)

hematology
histology

storage temp.

room temp

SMILES string

N([C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)O)C(=O)[C@@H](N)CCCCN

InChI

1S/C18H38N6O4/c19-10-4-1-7-13(22)16(25)23-14(8-2-5-11-20)17(26)24-15(18(27)28)9-3-6-12-21/h13-15H,1-12,19-22H2,(H,23,25)(H,24,26)(H,27,28)/t13-,14-,15-/m0/s1

InChI key

WBSCNDJQPKSPII-KKUMJFAQSA-N

Related Categories

Application

Poly-L-lysine solution has been used for the attachment of endothelial cell cross sections to UV-crosslinked PET membrane frame slides. Poly-L-lysine coated slides were used for the placement of tissue sections for immunohistochemical assay.
Poly-L-lysine polymers can be used in promoting cell adhesion to solid substrates, conjugation to methotrexate for increased drug transport, microencapsulation of islets, cell microencapsulation technology, microarray glass slide coating, and chromosomal preparations. Lower molecular weight poly-L-lysine (30,000-70,000) is less viscuous in solution, but higher molecular weight versions provide more attachment sites per molecule.

Biochem/physiol Actions

Poly-L-lysine is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. When it is absorbed to the cell culture surface, poly-L-lysine functions to increase the number of positively charged sites available for cell binding. With cells that can digest poly-L-lysine, poly-D-lysine should be used as the attachment factor.

Preparation Note

This product is supplied as a 0.10% (w/v) solution in water. Coating slides with this solution should be followed with a 5 minute incubation and drying the slides at room temperature. Coated slides will be stable for one year if protected from dust.

Other Notes

Poly-L-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-L-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the beta structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties.

Storage Class

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000471636
0000471635
0000471636
0000471635
0000405469

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Hermann C Altmeppen et al.
Molecular neurodegeneration, 6, 36-36 (2011-05-31)
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Kuczkowski J et al.
American Journal of Otolaryngology, 31, 404-404 (2010)
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Product Information Sheet - P8920

Global Trade Item Number

SKUGTIN
P8920-500ML04061838262134
P8920-100ML04061838262127

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