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P8920

Sigma-Aldrich

Poly-L-lysine solution

0.1 % (w/v) in H2O

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Synonym(s):
2-(4-Cyanoanilino)-4,6-dichloropyrimidine, 4-[(4,6-Dichloro-2-pyrimidinyl)amino]benzonitrile
CAS Number:
25988-63-0
MDL number:
MFCD00165424
eCl@ss:
32160406
NACRES:
NA.47

biological source

synthetic

Quality Level

200

shelf life

Expiry date on the label.

mol wt

150,000-300,000

concentration

0.1 % (w/v) in H2O

color

colorless

application(s)

hematology
histology

storage temp.

room temp

InChI

1S/C18H38N6O4/c19-10-4-1-7-13(22)16(25)23-14(8-2-5-11-20)17(26)24-15(18(27)28)9-3-6-12-21/h13-15H,1-12,19-22H2,(H,23,25)(H,24,26)(H,27,28)/t13-,14-,15-/m0/s1

InChI key

WBSCNDJQPKSPII-KKUMJFAQSA-N

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Application

Poly-L-lysine solution has been used for the attachment of endothelial cell cross sections to UV-crosslinked PET membrane frame slides. Poly-L-lysine coated slides were used for the placement of tissue sections for immunohistochemical assay.
Poly-L-lysine polymers can be used in promoting cell adhesion to solid substrates, conjugation to methotrexate for increased drug transport, microencapsulation of islets, cell microencapsulation technology, microarray glass slide coating, and chromosomal preparations. Lower molecular weight poly-L-lysine (30,000-70,000) is less viscuous in solution, but higher molecular weight versions provide more attachment sites per molecule.

Biochem/physiol Actions

Poly-L-lysine is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. When it is absorbed to the cell culture surface, poly-L-lysine functions to increase the number of positively charged sites available for cell binding. With cells that can digest poly-L-lysine, poly-D-lysine should be used as the attachment factor.

Components

Poly-L-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-L-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the beta structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties.

Preparation Note

This product is supplied as a 0.10% (w/v) solution in water. Coating slides with this solution should be followed with a 5 minute incubation and drying the slides at room temperature. Coated slides will be stable for one year if protected from dust.

replaced by

Product No.
Description
Pricing

Pictograms

Exclamation markEnvironment
GHS07,GHS09

Signal Word

Warning

Hazard Statements

H315 - H317 - H319 - H411

Hazard Classifications

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. Is Product P8920, Poly-L-lysine solution, recommended for cell culture use?

    No, the product contain 0.01% thimerosal as a preservative. For cell culture use, product P4832 is recommended.  This solution has the same molecular weight as P8920 but contains no preservative.  P4832 is sterile filtered and is considered suitable for cell culture use.

  4. What are the recommended uses for Product P8920, Poly-L-lysine solution?

    P8920 was developed for use in coating slides intended for use in either histology or immunohistochemistry.  Poly-L-lysine is preferred for uses such as silver staining procedures that require an alkaline pH.  Silane coated slides may be more effective is a low pH is required.

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