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Sigma-Aldrich

SIGMAFAST™ OPD

tablet

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Synonym(s):
OPD Peroxidase Substrate
NACRES:
NA.83

form

tablet

solubility

water: 0.5 tablet/10 mL, clear, colorless

storage temp.

2-8°C

Related Categories

Application

SIGMAFAST™ OPD (o-Phenylenediamine dihydrochloride) tablets have been developed for use as a soluble substrate for the detection of peroxidase activity in enzyme immunoassays (EIA). OPD is the EIA substrate of choice as it exhibits high sensitivity. EIA applications utilizing OPD may be read in timed assays or stopped with dilute acid solutions for delayed readings.
SIGMAFAST OPD has been used in saliva- virus-like particles (VLP) binding assay. It has also been used to develop enzyme linked immunosorbent assay (ELISA) plates for the detection of rituximab in rat serum samples.

Features and Benefits

SIGMAFAST™ OPD Tablets require no additional buffers or steps to prepare an active substrate solution.

Caution

This substrate is hygroscopic and light sensitive

Reconstitution

Each tablet set dissolved in 20 mL deionized water yields a ready-to-use buffered solution containing OPD and urea hydrogen peroxide.
One OPD tablet and one urea hydrogen peroxide/buffer tablet, dissolved in 20 ml of water, provides 20 ml of ready-to-use substrate. When the tablet is dissolved in this manner, the final concentrations will be 0.4 mg/ml OPD, 0.4 mg/ml urea hydrogen peroxide, and 0.05 M phosphate-citrate, pH 5.0. Tablets are individually packaged in foil packets.

Legal Information

SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Muta. 2 - Skin Sens. 1 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

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Subcutaneous absorption of monoclonal antibodies: role of dose, site of injection, and injection volume on rituximab pharmacokinetics in rats
Kagan L, et al.
Pharmaceutical Research, 29(2), 490-499 (2012)
Elisa Lobato et al.
Ecology and evolution, 7(11), 3645-3656 (2017-06-16)
Oceanic islands share several environmental characteristics that have been shown to drive convergent evolutionary changes in island organisms. One change that is often assumed but has seldom been examined is the evolution of weaker immune systems in island species. The
Jeffrey D Whitman et al.
Nature biotechnology, 38(10), 1174-1183 (2020-08-29)
Appropriate use and interpretation of serological tests for assessments of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) exposure, infection and potential immunity require accurate data on assay performance. We conducted a head-to-head evaluation of ten point-of-care-style lateral flow assays (LFAs)
Eliana Spilioti et al.
Environmental geochemistry and health, 39(4), 821-833 (2016-07-23)
Spa resorts are known for thousands of years for their healing properties and have been empirically used for the treatment of many inflammatory conditions. Mud is one of the most often used natural materials for preventive, healing and cosmetic reasons
Courtney Woolsey et al.
Nature immunology, 22(1), 86-98 (2020-11-26)
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is responsible for an unprecedented global pandemic of COVID-19. Animal models are urgently needed to study the pathogenesis of COVID-19 and to screen vaccines and treatments. We show that African green monkeys (AGMs)
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Articles

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

Protocols

ELISA Procedures

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