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Merck
CN

P9827

Polycytidylic acid–Agarose

lyophilized powder

Synonym(s):

Poly(C)–Agarose potassium salt

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About This Item

UNSPSC Code:
41106500
NACRES:
NA.56
MDL number:
Technical Service
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form

lyophilized powder

Quality Level

extent of labeling

0.25-1.0 mg per mL

matrix

cross-linked 4% beaded agarose

matrix activation

cyanogen bromide

matrix attachment

amino

matrix spacer

1 atom

swelling

1 g swells to ~5 mL

storage temp.

−20°C

Application

Polycytidylic acid-agarose is used in protein chromatography, affinity chromatography and specialy resins. Polycytidylic acid has been used to study the preferential induction of apoptosis in the rainbow trout macrophage cell line. Polycytidylic acid has also been used to develop two analytical methods for human secretory-type ribonuclease.

Physical form

Lyophilized powder stabilized with lactose


Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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D Nadano et al.
Analytical biochemistry, 212(1), 111-116 (1993-07-01)
Two analytical methods for human secretory-type ribonuclease, which are based on polycytidylic acid/ethidium bromide fluorescence, have been developed. The first is a method for measurement of secretory-type ribonuclease activity utilizing the radial diffusion of ribonuclease in a thin agarose gel
S J Dewitte-Orr et al.
Fish & shellfish immunology, 18(4), 279-295 (2004-11-25)
The rainbow trout macrophage cell line RTS11 was found to be considerably more sensitive than rainbow trout fibroblast (RTG-2) and Chinook salmon epithelial (CHSE-214) cell lines to killing by macromolecular synthesis inhibitors, actinomycin D (AMD) and cycloheximide (CHX), a synthetic
Preparation of sepharose-bound poly (rI:rC).
A F Wagner et al.
Biochemical and biophysical research communications, 45(1), 184-189 (1971-10-01)



Global Trade Item Number

SKUGTIN
P9827-1G04061826695302
P9827-250MG04061833290231