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PF0100

Protease Fluorescent Detection Kit

Name: Protease Fluorescent Detection Kit
Brand: SIGMA

high sensitivity assay

Synonym(s):

Protease Assay Kit

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About This Item

NACRES:

NA.26

UNSPSC Code:

12352202

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Quality Level

200

shipped in

dry ice

storage temp.

−20°C

Related Categories

Biochemicals

Metabolism Assay Kits

Application

Protease Fluorescent Detection Kit has been used:

in the detection of proteolytic activity using casein based protease assay
for testing the extracellular proteolytic activity of fungal strains
for quantifying mitochondrial protease activity
for measuring the protease production from soil solution

Fluorescent Protease Detection Kit Video Protocol

Detection limit: approx. 50 ng trypsin

Preparation Note

Supplied with sufficient reagents for up to 200 one mL assays.

The trichloroacetic acid (TCA) component is provided as 6.1 N TCA solution. 0.6 N TCA Solutions may be prepared by a 10-fold dilution of the desired volume of T0699 with ultrapure water. It is not necessary to dilute the entire volume of T0699 for immediate use of the kit.

Analysis Note

Tested for detection of all four proteolytic classes (serine, apartic, cysteine and metalloproteinases).

Kit Components Only

Product No.

Description

Incubation buffer 25 mL

FITC-casein solution 5 mL

Assay buffer 200 mL

Protease standard (trypsin) 20 μg

FITC control 50 mg

Trichloroacetic acid (TCA) solution 30 mL

pictograms

GHS08,GHS05,GHS07,GHS09

signalword

Danger

hcodes

H314,H317,H334,H335,H410

pcodes

P261 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338

target_organs

Respiratory system

Storage Class

8A - Combustible corrosive hazardous materials

flash_point_f

Not applicable

flash_point_c

Not applicable

wgk

WGK 3

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1 - Skin Sens. 1 - STOT SE 3

Regulatory Information

低风险生物材料

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Stimulation of soil organic nitrogen pool: The effect of plant and soil organic matter degrading enzymes

Kieloaho AJ, et al.

Soil Biology and Biochemistry, 96(10), 97-106 (2016)

Biological roles of the Podospora anserina mitochondrial Lon protease and the importance of its N-domain.

Céline Adam et al.

PloS one, 7(5), e38138-e38138 (2012-06-14)

Mitochondria have their own ATP-dependent proteases that maintain the functional state of the organelle. All multicellular eukaryotes, including filamentous fungi, possess the same set of mitochondrial proteases, unlike in unicellular yeasts, where ClpXP, one of the two matricial proteases, is

Dramatic increase in stability and longevity of enzymes attached to monodispersive iron nanoparticles.

Sharma A, et al.

IEEE Transactions on Magnetics, 43, 2418-2420 (2007)

Complement opsonization of HIV-1 results in a different intracellular processing pattern and enhanced MHC class I presentation by dendritic cells.

Veronica Tjomsland et al.

European journal of immunology, 43(6), 1470-1483 (2013-03-26)

Induction of optimal HIV-1-specific T-cell responses, which can contribute to controlling viral infection in vivo, depends on antigen processing and presentation processes occurring in DCs. Opsonization can influence the routing of antigen processing and pathways used for presentation. We studied

Limiting protease production plays a key role in the pathogenesis of the divergent clinical isolates of Staphylococcus aureus LAC and UAMS-1.

Joseph S Rom et al.

Virulence, 12(1), 584-600 (2021-02-05)

Using the USA300, methicillin-resistant Staphylococcus aureus strain LAC, we previously examined the impact of regulatory mutations implicated in biofilm formation on protease production and virulence in a murine sepsis model. Here we examined the impact of these mutations in the

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Protocols

Protease Fluorescent Detection Kit Usage

Protease Fluorescent Detection Kit detects protease activity using FITC-labeled casein substrate.

Global Trade Item Number

SKU	GTIN
PF0100-1KT	04061834413271

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