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PLA0210

Sigma-Aldrich

Rabbit anti-GRLF1/p190RhoGAP Antibody, Affinity Purified

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Synonym(s):
GRF-1, KIAA1722, rho GAP p190A, GRLF1, glucocorticoid receptor DNA binding factor 1, p190ARhoGAP, glucocorticoid receptor repression factor 1, p190RhoGAP, glucocorticoid receptor DNA-binding factor 1, P190-A, P190A

biological source

rabbit

Quality Level

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

grade

Powered by Bethyl Laboratories, Inc.

species reactivity

mouse, human

technique(s)

immunoprecipitation (IP): 2- 5 μg/mg
western blot: 1:2,000-1:10,000

accession no.

EAW57452.1

shipped in

wet ice

storage temp.

2-8°C

Gene Information

Immunogen

The epitope recognized by PLA0210 maps to a region between residue 1200 and 1250 of human glucocorticoid receptor DNA binding factor 1 (p190RHOGAP) using the numbering given in entry EAW57452.1 (GeneID 2909).

Physical form

Tris-buffered Saline containing 0.1% BSA containing 0.09% Sodium Azide

Other Notes

GRLF1 (glucocorticoid receptor DNA binding factor 1) was first identified as a factor that binds to the regulatory region of the human glucocorticoid receptor (GR) gene and represses GR transcription. Later, p190RhoGAP was identified as a protein that associates with p120RasGAP and through sequence comparison, p190RhoGAP and GRLF1 were determined to be one and the same. GRLF1/P190RhoGAP functions as a GTPase activating protein (GAP) for the Rho GTPase and plays an essential role in mediating cytoskeletal reorganization in response to growth factor signals. p190RhoGAP possesses an unusual GTP binding domain that may serve to regulate its GAP activity and the subsequent modulation of RhoGTPase activity. P190RhoGAP forms a significant complex with p120RasGAP. This association has been suggested to represent a method for the coordination of the Ras and Rho signaling pathways.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Haohui Duan et al.
The Journal of cell biology, 219(6) (2020-05-12)
BRCA1/2 help maintain genomic integrity by stabilizing stalled forks. Here, we identify the E3 ligase RFWD3 as an essential modulator of stalled fork stability in BRCA2-deficient cells and show that codepletion of RFWD3 rescues fork degradation, collapse, and cell sensitivity

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