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PP0510

GlycoProfile™ IV Chemical Deglycosylation Kit

TFMS Deglycosylation System

Synonym(s):

Deglycosylation kit

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About This Item

NACRES:
NA.32
UNSPSC Code:
12352200

Key Documents

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storage temp.

2-8°C

Quality Level

200

Related Categories

General description

The GlycoProfile™ IV Kit utilizes trifluoromethane sulfonic acid (TFMS) in a deglycosylation system that completely removes all N- and O-linked glycans while safeguarding the protein or polypeptide structure. TFMS hydrolysis of glycoproteins results in minimal protein degradation while the released glycans are eliminated. The conditions are optimized to deglycosylate simple or low molecular weight glycoproteins in 30 mins without the scavenger. For high molecular weight or complex, non-mammalian glycoproteins, anhydrous anisole is provided as a scavenger to give a high protein yield.

Application

GlycoProfile™ IV Chemical Deglycosylation Kit has been used in the deglycosylation of human α-L-iduronidase and peritrophic matrix from the gut of the caterpillar, Helicoverpa armigera. It has also been used to estimate the glycosylation of plant-derived recombinant human interleukin-13 (rhIL-13).

Features and Benefits

  • Eliminates both N- and O-linked glycans from proteins
  • This enables sequence analysis of the protein core by mass spectrometry (MS) or DNA-to-protein sequence comparison
  • Eliminates glycans which affect the molecular radius
  • Allows molecular mass determination by electrophoresis
  • Complete deglycosylation in as short as 30 mins for high throughput
  • Quick as it eliminates the need for multiple enzyme reactions
  • Accommodates a broad range of glycoproteins
  • Minimal degradation of the protein core to facilitate reliable MS results

Preparation Note

The kit contains sufficient reagents for 10 reactions with a sample size between 1-2 mg of a typical glycoprotein. An RNase B glycoprotein standard is included.

Legal Information

GlycoProfile is a trademark of Sigma-Aldrich Co. LLC

Kit Components Only

Product No.
Description
  • Trifluoromethanesulfonic Acid (TFMS) 5 x 1
  • RNase B Glycoprotein Standard 3 x 1
  • Pyridine solution, 60% 10 mL
  • Bromophenol blue solution, 0.2% .5 mL
  • Anisole, anhydrous 5 x 1
  • Reaction vials 10 ea

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Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 2 - Met. Corr. 1 - Resp. Sens. 1 - Skin Corr. 1B - STOT SE 1 - STOT SE 3

target_organs

Eyes,Central nervous system, Respiratory system

Storage Class

3 - Flammable liquids

Regulatory Information

危险化学品
低风险生物材料
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Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000380791
0000340860
0000340860
0000380791
SLCR4112

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A novel platform for biologically active recombinant human interleukin-13 production
David J W, et al.
Plant Biotechnology (2008)
Production and characterization of soluble human lysosomal enzyme alpha-iduronidase with high activity from culture media of transgenic tobacco BY-2 cells
Fu L H, et al.
Plant Science, 177(6), 668-675 (2009)
Proteomic analysis of the peritrophic matrix from the gut of the caterpillar, Helicoverpa armigera
Peter M C, et al.
Insect Biochemistry and Molecular Biology, 950-958 (2008)

Articles

Glycoprotein Deglycosylation

Information about Glycoprotein deglycosylation. The diversity of oligosaccharide structures, both O-linked and N-linked, often results in heterogeneity in the mass and charge of glycoproteins.

Protocols

Mass Spectrometry of Glycans

Explore mass spectrometry analysis of glycans for glycomic & glycoproteomic neutral & acidic glycan analysis. See a general mass spec glycan analysis procedure.

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