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PROTDT

Millipore

ProteoPrep® Detergent Sample Kit

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Synonym(s):
protein solubilization detergent
NACRES:
NA.32

shelf life

2 yr

Quality Level

200

storage temp.

15-25°C

Related Categories

General description

The ProteoPrep® Detergent Sample Kit provides ten detergents, non-ionic and zwitterionic, for protein solublization. The variety of innovative detergents enables optimization of extraction methods, which may vary with the protein of interest and cell type. Zwitterionic detergents uniquely offer some intermediate class properties that are superior to other detergent types. Each detergent is supplied in a convenient package size, ideal for method development and experimentation.

Application

ProteoPrep® Detergent Sample Kit has been used as a component of sample buffer for hepatoblastoma (HB) HepG2 cells.

Legal Information

ProteoPrep is a registered trademark of Merck KGaA, Darmstadt, Germany

Kit Components Only

Product No.
Description
  • Brij® C10 1 g

Kit Components Also Available Separately

Product No.
Description
SDS
  • A1346ASB-14 1 gSDS
  • C0856C7BzO 1 gSDS
  • P2393Polyoxyethylene (10) tridecyl ether, mixture of C11 to C14 iso-alkyl ethers with C13 iso-alkyl predominating 1 gSDS
  • C9426CHAPS hydrate, BioReagent, suitable for electrophoresis, ≥98% (HPLC) 1 gSDS
  • D4641n-Dodecyl β-D-maltoside, ≥98% (GC) 1 gSDS
  • O8001Octyl β-D-glucopyranoside, ≥98% (GC) 1 gSDS
  • O6004Octyl β-D-1-thioglucopyranoside, ≥98.0% (GC) 1 gSDS
  • D42663-(Decyldimethylammonio)­propane­sulfonate inner salt, zwitterionic detergent 1 gSDS
  • T8532Triton™ X-100, suitable for electrophoresis 1 mLSDS
See All (9)

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Repr. 1B - Skin Irrit. 2

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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NOX1 supports the metabolic remodeling of HepG2 cells
Bertram K, et al.
Testing, 10(3), e0122002-e0122002 (2015)
Katharina Bertram et al.
PloS one, 10(3), e0122002-e0122002 (2015-03-26)
NADPH oxidases are important sources of reactive oxygen species (ROS) which act as signaling molecules in the regulation of protein expression, cell proliferation, differentiation, migration and cell death. The NOX1 subunit is over-expressed in several cancers and NOX1 derived ROS

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