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ProteoPrep® Total Extraction Sample Kit
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About This Item
NACRES:
NA.32
UNSPSC Code:
12352200
Application
ProteoPrep® Total Extraction Sample Kit has been used to extract total protein from various sources such as epidermal stem cells and rheumatoid arthritis (RA) synovial fibroblasts.
Disclaimer
Samples containing high amount of salts and buffers may not work with this kit. These samples should be dialyzed prior to use. This product is for R&D use only, not for drug, household, or other uses.
Features and Benefits
- Four pre-mixed solubilization reagents - Enables rapid solubilization.
- Pre-measured reducing and alkylating reagents - Easy-to-use reagents provide improved IEF resolution.
- Innovative detergent preparations - Highly improved solubility allows higher protein loads and greater visibility of low abundance proteins in 2D gels.
General description
ProteoPrep® Total Extraction Sample Kit is designed to extract all proteins from any type of cell or tissue. It includes two reagents that have been used extensively for extraction and two new reagents with increased solubilizing power to extract more proteins. This kit also includes reagents for the reduction and alkylation of disulfide bonds. This allows comparison of the protein extractions obtained with each of the four reagents and optimization to meet your individual needs. The special reducing and alkylating reagents produce protein samples that exhibit improved focusing and decreased streaking in 2D gels.
Other Notes
The ProteoPrep® Total Extraction Kit was designed through a collaboration of Proteome Systems and Sigma research scientists.
This kit contains enough reagents to generate at least ten 2-ml samples using all four reagents. For researchers who have optimized an extraction protocol using one chaotropic extraction reagent, each reagent is available as an individual product as well.
This kit contains enough reagents to generate at least ten 2-ml samples using all four reagents. For researchers who have optimized an extraction protocol using one chaotropic extraction reagent, each reagent is available as an individual product as well.
Preparation Note
These reagents should remain stable for at least 6 months in their unopened container.
Legal Information
ProteoPrep is a registered trademark of Merck KGaA, Darmstadt, Germany
signalword
Danger
Hazard Classifications
Acute Tox. 3 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Repr. 1B - Resp. Sens. 1 - Skin Corr. 1A - Skin Sens. 1 - STOT SE 3
target_organs
Respiratory system
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
flash_point_f
Not applicable
flash_point_c
Not applicable
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Tsuyoshi Nakanishi et al.
Journal of biomolecular techniques : JBT, 18(5), 278-286 (2008-01-02)
We have developed a method for on-membrane direct identification of phosphoproteins, which are detected by a phosphate-binding tag (Phos-tag) that has an affinity to phosphate groups with a chelated Zn2+ ion. This rapid profiling approach for phosphoproteins combines chemical inkjet
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Canadian journal of microbiology, 57(4), 295-302 (2011-04-16)
A gram-negative, moderately halophilic bacterium was isolated from Çamaltı Saltern area, located in the Aegean Region of Turkey. Analysis of its 16S rRNA gene sequence and physiological characteristics showed that this strain belonged to the genus Halomonas ; hence, it
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