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R128

Monoclonal Anti-Ryanodine Receptor antibody produced in mouse

clone C3-33, ascites fluid

Synonym(s):

Anti-CCO, Anti-KDS, Anti-MHS, Anti-MHS1, Anti-PPP1R137, Anti-RYDR, Anti-RYR, Anti-RYR-1, Anti-SKRR

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
MFCD00282461
Conjugate:
unconjugated
Clone:
C3-33, monoclonal
Application:
immunohistochemistry (frozen sections)
immunoprecipitation (IP)
western blot (chemiluminescent)
Species reactivity:
canine, rabbit, rat, chicken, amphibian, guinea pig, mouse, fish, human
Citations:
11
Technique(s):
immunohistochemistry (frozen sections): 1 μg/mL using 4% paraformaldehyde-fixed, frozen mouse brain tissue
immunoprecipitation (IP): suitable
western blot (chemiluminescent): 1 μg/mL
Uniprot accession no.:
P21817

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Product Name

Monoclonal Anti-Ryanodine Receptor antibody produced in mouse, clone C3-33, ascites fluid

biological source

mouse

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

C3-33, monoclonal

mol wt

antigen ~565 kDa (non-mammalian vertebrates, a doublet at 565 kDa represents the α and β isoforms)

species reactivity

canine, rabbit, rat, chicken, amphibian, guinea pig, mouse, fish, human

concentration

~1 mg/mL

technique(s)

immunohistochemistry (frozen sections): 1 μg/mL using 4% paraformaldehyde-fixed, frozen mouse brain tissue
immunoprecipitation (IP): suitable
western blot (chemiluminescent): 1 μg/mL

isotype

IgG1

UniProt accession no.

P21817

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

rat ... Ryr1(114207), Ryr2(84025)

Application

Anti-Ryanodine Receptor has been used in immunofluorescence assay.

Biochem/physiol Actions

In rats PASMCs (pulmonary artery smooth muscle cells), RyR2 (ryanodine receptor isoform 1) participates in RyR-gated Ca2+ entry. It controls the secretion of insulin and glucose homeostasis. After hemorrhagic shock, RyR2 involves in the progress of vascular bi-phasic reactivity to NE (norepinephrine). In human, RyR1 plays an important role in muscle contraction. RYR1 mutation causes CCD (central core disease) and MHS (malignant hyperthermia susceptibility).
The antibody reacts strongly with RyR-2 (expressed predominantly in the heart muscle, but also found in stomach, endothelial cells and diffuse areas of the brain; also known as the β isoform), and weakly with RyR-1 (expressed predominantly in skeletal muscle and areas of the brain; also known as the α isoform). It reacts with ryanodine receptor in cardiac muscle of canine, rat, finch and pigeon; in visceral smooth muscle of toad; and in rat brain. It reacts with skeletal muscle of fish and the β isoform of frog, but only weakly with skeletal muscle in rabbit and the α isoform of frog. In immunohistochemistry, the antibody stains are consistent with the endoplasmic reticulum localization of the receptor in the hippocampus.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

RyR1 (ryanodine receptor isoform 1) is a homotetrameric intracellular calcium channel, that is present in skeletal muscle tissue. It is located on human chromosome 19q13.1. In rats, RyR1 and RyR2 are expressed in PASMCs (pulmonary artery smooth muscle cells).

Immunogen

dog cardiac ryanodine receptors (RyR)

Physical form

Containing PBS and 0.05% sodium azide

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Storage Class

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000513564
0000513564
0000465595
0000463026
0000463026

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Wei Shang et al.
Circulation research, 114(3), 412-420 (2013-11-22)
In cardiac dyads, junctional Ca2+ directly controls the gating of the ryanodine receptors (RyRs), and is itself dominated by RyR-mediated Ca2+ release from the sarcoplasmic reticulum. Existing probes do not report such local Ca2+ signals because of probe diffusion, so
Glucose-Dependent Insulin Secretion in Pancreatic ?-Cell Islets from Male Rats Requires Ca2+ Release via ROS-Stimulated Ryanodine Receptors
Llanos P, et al.
PLoS ONE, 10(6) (2015)
Conformation of ryanodine receptor-2 gates store-operated calcium entry in rat pulmonary arterial myocytes
Lin AH, et al.
Cardiovascular Research, 111(1), 94-104 (2016)
An autosomal dominant congenital myopathy with cores and rods is associated with a neomutation in the RYR1 gene encoding the skeletal muscle ryanodine receptor
Monnier N, et al.
Human Molecular Genetics, 9(18), 2599-2608 (2000)
Imaging Ca2+ nanosparks in heart with a new targeted biosensor
Shang W, et al.
Circulation Research, 114(3), 412-420 (2014)
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Data Sheet - R128

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