Key Documents

View All Documentation

R5500

Ribonuclease A from bovine pancreas

Name: Ribonuclease A from bovine pancreas
Brand: SIGMA

Type XII-A, ≥90% (SDS-PAGE), 75-125 Kunitz units/mg protein

Synonym(s):

Pancreatic Ribonuclease, RNAsea, RNase A, Ribonucleate 3′-pyrimidinooligonucleotidohydrolase

Select a Size

Change View

About This Item

CAS Number:

9001-99-4

NACRES:

NA.54

UNSPSC Code:

12352204

EC Number:

232-646-6

MDL number:

MFCD00132181

EC Number:

3.1.27.5 (BRENDA, IUBMB)

Specific activity:

75-125 Kunitz units/mg protein

Assay:

≥90% (SDS-PAGE)

Biological source:

bovine pancreas

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

Properties

biological source

bovine pancreas

Quality Segment

300

type

Type XII-A

assay

≥90% (SDS-PAGE)

form

lyophilized powder

specific activity

75-125 Kunitz units/mg protein

mol wt

~13,700

technique(s)

cell based assay: suitable

impurities

salt, essentially free

input

general use sample(s)

suitability

suitable for mRNA or total RNA extracted from cells and tissues

application(s)

diagnostic assay manufacturing

foreign activity

protease, essentially free

storage temp.

−20°C

SMILES string

[nH]1cnc(c1)CC(NC(=O)CCN)C(=O)O

InChI

1S/C9H14N4O3/c10-2-1-8(14)13-7(9(15)16)3-6-4-11-5-12-6/h4-5,7H,1-3,10H2,(H,11,12)(H,13,14)(H,15,16)

InChI key

CQOVPNPJLQNMDC-UHFFFAOYSA-N

Description

General description

RNase A, Ribonuclease A, is an endoribonuclease that cleaves the phosphodiester bonds of single strand RNA after pyrimidine nucleotides. It attacks at the 3′ phosphate end (For example pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG). The highest activity is exhibited with single stranded RNA. RNase A is a single chain polypeptide containing 4 disulfide bridges. In contrast to RNase B, it is not a glycoprotein. Ribonucleases do not hydrolyze DNA, because the DNA lacks 2′-OH groups essential for the formation of cyclic intermediates. RNase A can also hydrolyze RNA from protein samples. RNase A can be inhibited by alkylation of His12 and His119 and activated by potassium and sodium salts. RNAse is inhibited in the presence of heavy metal ions. RNase is also inhibited competitively by DNA.

Application

RNase A is used to remove RNA from DNA plasmid and genomic DNA preparations and protein samples.
RNase A is also used in RNA sequence analysis and protection assays.
RNase A has been used as a tool for computer-aided drug design.
RNase A supports the analysis of RNA sequences.
RNase A hydrolyze RNA contained in protein samples.
Purification of DNA is supported by RNase A.

Biochem/physiol Actions

Ribonuclease A is an endoribonuclease that cleaves single stranded RNA after pyrimidine nucleotides. It attacks at the 3′ phosphate end. Ribonucleases do not hydrolyze DNA, because the DNA lacks 2′-OH groups essential for the formation of cyclic intermediates. RNase can also hydrolyze RNA from protein samples. RNase A can be inhibited by alkylation of His12 and His119 and activated by potassium and sodium salts.

Features and Benefits

Our highly stable Ribonuclease A, RNase A, is suitable for removal of RNA, RNA sequencing, and DNA purification.

Preparation Note

Salt fractionated and chromatographically purified.

Analysis Note

Protein determined by E.

Still not finding the right product?

Explore all of our products under Ribonuclease A from bovine pancreas

Pictograms

GHS08

Signal Word

Danger

Hazard Codes

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)