R5506
Anti-Rabbit IgG (whole molecule) antibody produced in goat
IgG fraction of antiserum, buffered aqueous solution
Synonym(s):
Rabbit IgG Antibody
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About This Item
Conjugate:
unconjugated
Clone:
polyclonal
Application:
indirect ELISA
quantitative precipitin assay
quantitative precipitin assay
Technique(s):
indirect ELISA: 1:30,000
quantitative precipitin assay: 3.0-4.5 mg/mL
quantitative precipitin assay: 3.0-4.5 mg/mL
Citations:
22
Product Name
Anti-Rabbit IgG (whole molecule) antibody produced in goat, IgG fraction of antiserum, buffered aqueous solution
biological source
goat
conjugate
unconjugated
antibody form
IgG fraction of antiserum
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous solution
technique(s)
indirect ELISA: 1:30,000
quantitative precipitin assay: 3.0-4.5 mg/mL
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
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Application
Anti-Rabbit IgG (whole molecule) antibody is suitable for use in quantitative precipitin assay, immunoelectrophoresis, immunohistochemistry , and laser scattering microscopy .
Anti-Rabbit IgG (whole molecule) antibody produced in goat has been used as secondary antibody in vivo xenograft assay in non-small-cell lung cancer (NSCLC) tissues and in western blotting detection in endometrial sample from cattle.
Biochem/physiol Actions
Anti-Rabbit IgG (whole molecule) antibody is specific for IgGs in rabbits.
Digestion of IgG by papain results in the generation of fragment antigen binding (Fab). Pepsin digestion of IgG produces fragment crystallizable (Fc). The Fc region of IgG antibody has enormous therapeutic potential and is exploited for the development of therapeutic antibodies. IgG1 class is the most abundant and its deficiency results in hypogammaglobulinemia. IgG2 deficiency increases susceptibility to bacterial infections. IgG3 mediates effector functions, and IgG4 is associated with asymptomatic infection.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Immunoglobulins (Igs) belong to the immunoglobulin super-family and have two heavy (H) and two light (L) chains, held together by disulfide linkages. The heavy chain has one variable N-terminal region and three to four constant (CH1-CH4) C-terminal regions. The L chain comprises of one variable N-terminal region and a constant C-terminal region. IgG is an abundant protein in human serum. The four classes of IgG include IgG1, IgG2, IgG3 and IgG4. The IgG heavy chain region is mapped to human chromosome 14.
Rabbit immunoglobulins (IgGs) are often used as primary antibodies in several protein assays. Thus, anti-rabbit IgGs can be used as secondary antibodies to study and characterize target proteins bound to rabbit IgGs. Anti-Rabbit IgG (whole molecule) antibody is specific for IgGs in rabbits.
Immunogen
Rabbit IgG
Physical form
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as preservative
Preparation Note
For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
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Storage Class
10 - Combustible liquids
Regulatory Information
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J Yuan et al.
Experimental mechanics, 50(9) (2010-11-01)
At room temperature at stall, the flagellar motor of the bacterium Escherichia coli exerts a torque of ~1300 pN nm. At zero external load, it spins ~330 Hz. A robust method for studying the motor near zero load is reviewed
E F Kirkness et al.
The Biochemical journal, 256(1), 291-294 (1988-11-15)
A synthetic peptide, corresponding to amino acid residues 101-109 of the bovine gamma-aminobutyrate/benzodiazepine receptor alpha-subunit, was used to raise a polyclonal antiserum. The reactivity of this antiserum towards polypeptides of both bovine and pig receptor preparations was established by immunoprecipitation
R Pippa et al.
Oncogene, 31(38), 4207-4220 (2011-12-20)
The cyclin-cdk (cyclin-dependent kinase) inhibitor p27Kip1 (p27) has a crucial negative role on cell cycle progression. In addition to its classical role as a cyclin-cdk inhibitor, it also performs cyclin-cdk-independent functions as the regulation of cytoskeleton rearrangements and cell motility.
Jisheng Peng et al.
Biomedical chromatography : BMC, 36(9), e5414-e5414 (2022-05-24)
Xiaopi granules have been shown to ameliorate gastric epithelial dysplasia in patients. However, the therapeutic mechanism is unclear. Herein, the proteomics method was applied to identify the differentially expressed proteins and related pathways. Sixty male Sprague-Dawley rats were randomly divided
Steven Johnson et al.
Nature microbiology, 6(6), 712-721 (2021-05-02)
The bacterial flagellum is a macromolecular protein complex that enables motility in many species. Bacterial flagella self-assemble a strong, multicomponent drive shaft that couples rotation in the inner membrane to the micrometre-long flagellar filament that powers bacterial swimming in viscous
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