R5509
Nde I from Neisseria denitrificans
Restriction Enzyme
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About This Item
grade
Molecular Biology
form
buffered aqueous glycerol solution
concentration
10,000 units/mL
shipped in
wet ice
storage temp.
−20°C
Application
NdeI is a restriction endonuclease used in molecular biology methods to cleave DNA at the recognition site 5′-CA/TATG-3′, generating fragments with 5′-cohesive ends.
Biochem/physiol Actions
Recognition sequence: 5′-CA/TATG-3′
Cutting results: a 2-10-fold Nde I overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: Inactivated at 60 °C for 15 minutes.
Cutting results: a 2-10-fold Nde I overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: Inactivated at 60 °C for 15 minutes.
Physical form
Solution in 20 mM Tris-HCl, pH 7.5, 0.1 mM EDTA, 100 mM NaCl, 1 mM dithioerythritol, 0.02% polydocanol, 0.01% gelatine, 50% glycerol (v/v) at 4 °C.
Other Notes
One unit is the enzyme activity that completely cleaves 1 mg λDNA in 1 hr. at 37 °C in a total volume of 25 mL of restriction endonuclease buffer SH.
Supplied with 10x Restriction Enzyme Buffer SH (B3657).
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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