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R6400

Sigma-Aldrich

Anti-phospho-Retinoblastoma (pSer807,811) antibody produced in rabbit

affinity isolated antibody, buffered aqueous glycerol solution

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About This Item

MDL number:
MFCD02097167
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous glycerol solution

species reactivity

rat, mouse, human

technique(s)

western blot (chemiluminescent): 1:1,000 using human fibroblasts synchronized by serum deprivation

UniProt accession no.

P06400

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

phosphorylation (pSer807/pSer811)

Gene Information

human ... RB1(5925)
mouse ... Rb1(19645)
rat ... Rb1(24708)

General description

RB1 (RB transcriptional corepressor 1) codes for nuclear phosphoprotein pRB (retinoblastoma protein). It is located on human chromosome 13q14. It is a tumor-suppressor gene.

Specificity

Recognizes phosphorylated Rb (Ser807/Ser811) and does not react with non-phosphorylated Rb. Does not react with Rb phosphorylated at other sites.

Immunogen

synthetic peptide corresponding to residues around phosphoserine 807/811 of the human retinoblastoma tumor suppressor protein (Rb), conjugated to KLH.

Application

Anti-phospho-Retinoblastoma (pSer807,811) has been used in western blotting.

Biochem/physiol Actions

RB1 (RB transcriptional corepressor 1) plays an important role throughout G1/S phase transition. Absence of RB induces prostate cancer metastasis. It plays an important role in modulating the cell cycle. It also regulates cell stemness and differentiation.

Physical form

Solution in 10 mM sodium HEPES, pH 7.5, containing 150 mM sodium chloride, 100 μg/ml bovine serum albumin and 50% glycerol.

Preparation Note

Purified using protein A and peptide affinity chromatography.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
SLBS0110
SLBQ8197
118K1324
086K1370
103K1259

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