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R7017

Resazurin sodium salt

powder, BioReagent

Synonym(s):

7-Hydroxy-3H-phenoxazin-3-one-10-oxide sodium salt

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About This Item

Empirical Formula (Hill Notation):
C12H6NNaO4
CAS Number:
62758-13-8
Molecular Weight:
251.17
UNSPSC Code:
12352207
NACRES:
NA.75
PubChem Substance ID:
24899387
EC Number:
263-718-5
Beilstein/REAXYS Number:
3794971
MDL number:
MFCD00005036
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product line

BioReagent

Quality Level

200

form

powder

composition

Dye content, ~80%

pH

3.8-6.5, orange to violet

solubility

H2O: soluble

λmax

600 nm

SMILES string

[Na+].[O-]c1ccc2c(OC3=CC(=O)C=CC3=[N+]2[O-])c1

InChI

1S/C12H7NO4.Na/c14-7-1-3-9-11(5-7)17-12-6-8(15)2-4-10(12)13(9)16;/h1-6,14H;/q;+1/p-1

InChI key

IVGPGQSSDLDOLH-UHFFFAOYSA-M

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Application

Resazurin sodium salt has been used in viability assay:
  • to measure cell viability of A. fumigatus
  • to assess cell proliferation
  • to determine A. fumigatus viability in high-throughput screenings of microbial natural extracts

For the measurement of metabolic activity and proliferation of living cells. The bioreduction of the dye reduces the amount of the oxidized form (blue) and concomitantly increases the fluorescent intermediate (red).

Biochem/physiol Actions

Resazurin is a blue, non-fluorescent dye originally used to test milk/biological fluids for bacterial and yeast contamination. It is also useful in sperm viability and semen quality test. Resazurin is applicable in cytotoxicity determination.

pictograms

Exclamation mark
GHS07

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000541645
0000541645
SHBT5168
0000321963
0000305141

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More than 100 years after being first described, Chagas disease remains endemic in 21 Latin American countries and has spread to other continents. Indeed, this disease, which is caused by the protozoan parasite Trypanosoma cruzi, is no longer just a problem
Investigation of the Alamar Blue (resazurin) fluorescent dye for the assessment of mammalian cell cytotoxicity
O Brien J, et al.
European Journal of Biochemistry, 267(17), 5421-5426 (2000)
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Chao DT, et al.
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Global Trade Item Number

SKUGTIN
R7017-1G04061835569335
R7017-5G04061836695965

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