R7023
Pst I from Providencia stuartii
Restriction Enzyme
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About This Item
grade
Molecular Biology
form
buffered aqueous glycerol solution
concentration
10,000 units/mL
shipped in
wet ice
storage temp.
−20°C
Application
PstI is a restiction enzyme used in molecular biology applications to cleave DNA at the recognition sequence 5′-CTGCA/G-3′, generating DNA fragments with 3′- cohesive termini.
Biochem/physiol Actions
Recognition sequence: 5′-CTGCA/G-3′
Cutting results: a 2-10-fold Pst I overdigestion of 1 μg λ DNA substrate results in 100% cutting.
Heat inactivation: Inactivated at 80 °C for 20 minutes.
Cutting results: a 2-10-fold Pst I overdigestion of 1 μg λ DNA substrate results in 100% cutting.
Heat inactivation: Inactivated at 80 °C for 20 minutes.
Physical form
Solution in 10 mM Tris-HCl, pH 7.0, 1 mM EDTA, 250 mM NaCl, 1 mM 2-mercaptoethanol, 50% glycerol (v/v), 0.01% polydocanol (v/v) at 4 °C
Other Notes
Comment: Pst I requires optimal reaction conditions in order to avoid star activity.
Supplied with 10x Restriction Enzyme Buffer SH (B3657).
Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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T B Britschgi et al.
Applied and environmental microbiology, 57(6), 1707-1713 (1991-06-01)
The identification of the prokaryotic species which constitute marine bacterioplankton communities has been a long-standing problem in marine microbiology. To address this question, we used the polymerase chain reaction to construct and analyze a library of 51 small-subunit (16S) rRNA
Thomas Hiesberger et al.
The Journal of clinical investigation, 113(6), 814-825 (2004-04-07)
Hepatocyte nuclear factor-1beta (HNF-1beta) is a Pit-1, Oct-1/2, UNC-86 (POU)/homeodomain-containing transcription factor that regulates tissue-specific gene expression in the liver, kidney, and other organs. Humans with autosomal dominant mutations of HNF-1beta develop maturity-onset diabetes of the young type 5 (MODY5)
The mapping and sequence determination of the single site in phiX174am3 replicative form DNA cleaved by restriction endonuclease Pst I.
N L Brown et al.
FEBS letters, 65(3), 284-287 (1976-06-15)