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R7638

RPMI-1640 Medium

Name: RPMI-1640 Medium
Brand: SIGMA

Dutch Modification, with sodium bicarbonate and HEPES, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

Roswell Park Memorial Institute 1640 medium

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About This Item

UNSPSC Code:

12352207

NACRES:

NA.75

MDL number:

MFCD00217820

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Product Name

RPMI-1640 Medium, Dutch Modification, with sodium bicarbonate and 20mM HEPES, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Quality Level

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

pH

>7.2

components

sodium pyruvate: no
HEPES: 20 mM
L-glutamine: no
NaHCO₃: yes
phenol red: yes

shipped in

ambient

storage temp.

2-8°C

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Related Categories

Cell Culture & Analysis

Cell Culture Media & Buffers

Classical Media & Buffers

Molecular Biology & Functional Genomics

RPMI 1640 Media

General description

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.

Application

RPMI-1640 Medium has been used

to obtain spleen and lymph nodes from mice
for the resuspension of Staphylococcus aureus strains in the phagocytosis assay
in the isolation of hepatitis B surface antigen-specific B-cell clones

Preparation Note

Supplement with 0.3 g/L L-glutamine.

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

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Blood polymorphonuclear leukocyte responses against Staphylococcus aureus in primiparous and pluriparous Lacaune and Santa Ines ewes

Marques M, et al.

Small Ruminant Research, 201, 106412-106412 (2021)

Kamila R Santos et al.

Vaccines, 9(8) (2021-08-29)

Staphylococcus aureus mastitis remains a major challenge for dairy farming. Here, 24 mice were immunized and divided into four groups: G1: control; G2: Granulocyte Macrophage Colony-Stimulating Factor (GM-CSF) DNA vaccine; G3: F0F1 ATP synthase subunit α (SAS), succinyl-diaminopimelate (SDD), and

Milk lymphocyte profile and macrophage functions: new insights into the immunity of the mammary gland in quarters infected with Corynebacterium bovis.

Vitória M Silva et al.

BMC veterinary research, 17(1), 282-282 (2021-08-27)

The present study explored the viability of bovine milk macrophages, their intracellular production of reactive oxygen and nitrogen species (RONS), and their phagocytosis of Staphylococcus aureus, as well as the profile of lymphocytes, from healthy udder quarters and udder quarters

Milk Macrophage Function in Bovine Leukemia Virus-Infected Dairy Cows.

Ewerton de Souza Lima et al.

Frontiers in veterinary science, 8, 650021-650021 (2021-07-06)

The implications of bovine leukemia virus (BLV) on innate and adaptive immune responses have been widely investigated; however, the effects of BLV on mammary gland immunity require further investigation. The present study investigated the viability, phagocytic capacity, and intracellular production

A Human Monoclonal Antibody against Hepatitis B Surface Antigen with Potent Neutralizing Activity.

Antonella Cerino et al.

PloS one, 10(4), e0125704-e0125704 (2015-04-30)

We describe the production and characterization of human monoclonal antibodies (mAb) specific for the major hepatitis B virus (HBV) S protein. The mAbs, two IgG1κ and one IgG1λ, were secreted by B-cell clones obtained from peripheral blood mononuclear cells (PBMC)

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Global Trade Item Number

SKU	GTIN
R7638-6X500ML	04061836696573
R7638-500ML	04061836696542

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